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Qiagen 凯杰 Biosharp Omega

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GLP-1 Total ELISA Kit (BULK PK) CPACKS

市场价：
43.00
促销价：
¥0.00

货号：EZGLP1T-36BK

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications
Glucagon Like Peptide-1 H, M, R ELISA
Description
Catalogue Number EZGLP1T-36BK
Description Multi Species GLP-1 Total ELISA- Bulk
Background Information Glucagon-like peptide-1 (GLP-1) is a 30 amino acid gut hormone secreted from the intestinal L-cells. The GLP-1 sequence is highly conserved among mammals and is released in its active form (7-36) in response to food intake. It is quickly degraded into its inactive form (9-36) after cleavage by DPPIV. GLP-1 reduces glucose levels by regulating pancreatic secretion, slowing gastric emptying and lowering the desire for food intake. Therefore, it is an important biomarker for the study of diabetes and obesity. This kit measures both the active (7-36) and inactive (9-36) forms of GLP-1.
References
Product Information
Precision, %
  • Intra-assay: < 5%
  • Inter-assay:< 12%
Applications
Application This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.
Key Applications
  • ELISA
Application Notes This is a 3.5-hour assay at room temperature requiring 50 uL of serum, plasma or culture samples per well.
Biological Information
Specificity The antibody pair used in this assay is specific to GLP-1 (7-36) and (9-36) and has no cross-reactivity with GLP-2, GIP, Glucagon or Oxyntomodulin.
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • Glucagon Like Peptide-1
Cross Reactivity GLP-1 is conserved in almost all mammals.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Gene Symbol
  • GCG
  • GRPP
  • GLP1
  • GLP2
  • glucagon
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
Accuracy
  • Recovery: 90 - 110%
  • Linearity 90 - 132%
Physicochemical Information
Sensitivity
  • MinDC is 1.5 pM
Standard Curve Range
  • 4.1 – 1000 pM
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size Ten 96-well plates
Material Package This product consists of ten GLP-1 (total) ELISA kits.
Transport Information
Supplemental Information
Specifications

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