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GLP-1 (Active) ELISA Kit / 10 Plates (BULK PKG) COLD PACKS

市场价：
53.00
促销价：
¥0.00

货号：EGLP-35BK

品牌：Millipore 密理博

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Replacement Information
Description
Catalogue Number EGLP-35BK
Description Glucagon Like Peptide-1 (Active) Space Saver (Bulk) ELISA
Background Information GLP-1 is secreted from gut endocrine cells in response to nutrient ingestion and plays multiple roles in metabolic homeostasis.
References
Product Information
Detection method Fluorescent
Precision, %
  • Inter-assay: 8 ± 4.8
  • Intra-assay: 7.4 ± 1.1
Applications
Application This Glucagon Like Peptide-1 (Active) ELISA is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application Notes Overnight incubation at 4°C, followed by 2 hours 30 minutes at room temperature Sample volume 100 μl serum, plasma or tissue culture medium
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • Glucagon Like Peptide-1
Cross Reactivity GLP-1 is conserved in almost all mammals. This kit may be used to mearure in such species (e.g. rat, mouse, primates, etc.)
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Gene Symbol
  • GCG
  • GRPP
  • GLP1
  • GLP2
  • glucagon
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
SIZE: 180 amino acids; 20909 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
Accuracy
  • 86.9 ± 5.2%
Physicochemical Information
Standard Curve Range
  • 2–100 pM
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size Ten 96-well strip plates
Material Package This product contains 10 GLP-1 (Active) ELISA kits.
Transport Information
Supplemental Information
Specifications

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