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Qiagen 凯杰 Biosharp Omega

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Human Insulin ELISA Kit (Animal Serum Free)

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¥0.00

货号：EZHIASF-14K

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Insulin H ELISA Fluorescent, Colorimetric
Description
Catalogue Number EZHIASF-14K
Description Human Insulin ELISA, animal serum free
Background Information This unique Human Insulin Elisa kit is animal serum free–the monoclonal antibodies are harvested from clones grown in culture medium devoid of any animal protein.
References
Product Information
Detection method Fluorescent Colorimetric
Precision, %
  • Inter-assay: Coefficient of Variation:6.0–7.1
  • Intra-assay: Coefficient of Variation: 1.7–1.9
Applications
Application This Human Insulin ELISA, animal serum free is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application Notes 3.5 hour assay, room temperature, 20 µL sample volume
Biological Information
Specificity No Cross-reactivity with Human Pro-insulin or Human C-peptide
Species Reactivity
  • Human
Analytes Available
  • Insulin
Entrez Gene Number
Entrez Gene Summary After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 88–128%
Physicochemical Information
Sensitivity
  • 0.73 µU/mL
Linearity of Dilution 73–100%
Standard Curve Range
  • 2–200 µU/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size 96-well strip plate
Transport Information
Supplemental Information
Specifications

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