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Human Total/Intact Proinsulin ELISA Microtiter Plate

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货号：EP15

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M, R WB, IHC Rb Unpurified Monoclonal Antibody
Description
Catalogue Number MABT66
Description Anti-ILK Antibody, clone EP1593Y rabbit monoclonal
Alternate Names
  • integrin-linked kinase
  • P59
  • DKFZp686F1765
  • 59 kDa serine/threonine-protein kinase
  • p59ILK
Background Information Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3).
References
Product Information
Format Unpurified
Control
  • COS cell lysate
Presentation Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.05% BSA.
Applications
Application Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-ILK Antibody, clone EP1593Y Rabbit is an antibody against ILK for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application Notes Immunohistochemistry Analysis: 1:50-100 dilution from a previous lot detected ILK in human heart muscle.
Biological Information
Immunogen Synthetic peptide corresponding to residues near the N-terminus of human ILK.
Clone EP1593Y
Host Rabbit
Specificity The antibody recognizes ILK at the N-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity Note Proven to react with human, mouse, and rat.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ILK-1
  • ILK1
  • ILK-2
  • EC 2.7.11.1
  • ILK2
Purification Method Unpurified
UniProt Number
Molecular Weight ~ 51 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blot in COS cell lysate.
Western Blot Analysis: 1:2,000-10,000 dilution of the antibody was used to detect ILK in COS lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 100 µL
Transport Information
Supplemental Information
Specifications

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