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Qiagen 凯杰 Biosharp Omega

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Mouse SAA-3 Detection Antibody (ELISA) 11ml.

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货号：E1012

品牌：Millipore 密理博

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Replacement Information

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H, M WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number MABE1012
Description Anti-E2F-7 Antibody, clone 14C3.2
Alternate Names
  • Transcription factor E2F7
  • E2F-7
Background Information Transcription factor E2F7 (UniProt Q96AV8; also known as E2F-7) is encoded by the E2F7 gene (Gene ID 144455) in human. The E2F family of transcription factors (E2F) regulate genes involved in cell proliferation, differentiation and apoptosis. E2F1, 2, 3a function as transcription activators, while other family members (E2F3b, 4, 5, 6, 7, 8) are known as transcription repressors. Upregulated E2F7 is found in tamoxifen-resistant breast cancer cells, where it is responsible for transcriptional repression of miR-15a/16. Exogenous expression of miR-15a/16 or silencing E2F7 re-sensitizes breast cancer cells to tamoxifen-induced cell cycle arrest and apoptosis. Upregulated E2F7 expression is also reported in cutaneous squamous cell carcinomas (CSCC) and and head and neck SCC (HNSCC), where E2F7-dependent Sphk1 overexpression in SCC results in an elevated production of the antiapoptotic phospholipid, sphingosine-1-phosphate (S1P), which in turn invokes anthracycline resistance via activation of the PI3K/AKT pathway.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application This Anti-E2F-7 Antibody, clone 14C3.2 is validated for use in Western Blotting, Immunocytochemistry for the detection of E2F-7.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected E2F-7 in A431, HeLa, HepG2 and NIH/3T3 cells.
Biological Information
Immunogen GST-tagged recombinant human E2F-7 internal fragment.
Epitope Internal (C-terminal half).
Clone 14C3.2
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 14C3.2 recognizes an internal epitope of human E2F-7 present in all three spliced isoforms reported by UniProt (Q96AV8).
Isotype IgG2bκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity Note Human, Mouse.
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • E2F7
Purification Method Protein G purified
UniProt Number
Molecular Weight ~100 kDa observed. 99.89 kDa (isoform 1; E2F7b), 80.87 kDa (isoform 2; E2F7a), 98.35 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected E2F-7 in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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