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Recombinant Human KGF

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货号：GF008

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E.coli Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of KGF.
Description
Catalogue Number GF008
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Keratinocyte Growth Factor Protein, Recombinant human
Overview Keratinocyte Growth Factor (KGF or FGF-7) is a potent mitogen for keratinocytes and epithelial cells. Human KGF is a 18.9 kDa protein containing 163 amino acid residues.
Alternate Names
  • KGF
  • FGF-7
References
Product Information
Presentation Lyophilized from 15 mM Sodium Phosphate pH 7.0 + 0.25 M NaCl.
Applications
Application The Keratinocyte Growth Factor protein (KGF protein or FGF-7 protein) is a potent mitogen for keratinocytes & epithelial cells.
Key Applications
  • Cell Culture
Biological Information
Purity Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of KGF.
Source E.coli
Specific Activity The ED50 was determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is <= 10 ng/mL, corresponding to a specific activity of >= 1 x 105 units/mg.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.
Gene Symbol
  • FGF7
  • HBGF-7
  • FGF-7
  • KGF
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21781 # Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
SIZE: 194 amino acids; 22509 Da
SUBUNIT: Interacts with FGFBP1.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Epithelial cell.
SIMILARITY: SwissProt: P21781 ## Belongs to the heparin-binding growth factors family.
Stem Cell Type
  • Epithelial Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. After a quick spin, reconstitute in PBS to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted KGF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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