Recombinant Rat TNF-alpha

Key Applications	Species	Source	Purity
CULT	Rat	E.coli	Greater than 98% by SDS-PAGE and HPLC analysis

Key Applications

Species

Source

Purity

CULT

Rat

E.coli

Greater than 98% by SDS-PAGE and HPLC analysis

Description
Catalogue Number	GF046
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Tumor Necrosis Factor-α Protein, Recombinant rat
Overview	Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-alpha is a 17 kDa protein containing 157 amino acid residues.
Alternate Names	TNF-alpha

Description

Catalogue Number

GF046

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Tumor Necrosis Factor-α Protein, Recombinant rat

Overview

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Rat TNF-alpha is a 17 kDa protein containing 157 amino acid residues.

Alternate Names

TNF-alpha

Product Information
Presentation	Sterile filtered and lyophilized from 1x PBS. Contains no preservative.

Product Information

Presentation

Sterile filtered and lyophilized from 1x PBS. Contains no preservative.

Applications
Application	Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.
Key Applications	Cell Culture
Application Notes	For most in vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL.

Applications

Application

Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells & certain other target cells.

Key Applications

Cell Culture

Application Notes

For most in vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/mL.

Biological Information
Purity	Greater than 98% by SDS-PAGE and HPLC analysis
Source	E.coli
Entrez Gene Summary	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
UniProt Number	P01375 P16599
UniProt Summary	FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. SIZE: 233 amino acids; 25644 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted. PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation. SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
Stem Cell Type	Hematopoietic Stem Cells

Biological Information

Purity

Greater than 98% by SDS-PAGE and HPLC analysis

Source

E.coli

Entrez Gene Summary

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

UniProt Number

UniProt Summary

FUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
SIZE: 233 amino acids; 25644 Da
SUBUNIT: Homotrimer.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.

Stem Cell Type

Hematopoietic Stem Cells

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging Information
Material Size	20 µg

Packaging Information

Material Size

20 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Recombinant Rat TNF-alpha

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