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Rac1 Activation Assay Kit (non-radioactive)

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货号：17-283

品牌：Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications
H, M, R ABA
Description
Catalogue Number 17-283
Replaces SGT425
Brand Family Upstate
Trade Name
  • Upstate
Description Rac1 Activation Assay Kit
References
Product Information
Components
  • Anti-Rac1, clone 23A8 (Cat.# 05-389)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
  • Rac/cdc42 Assay Reagent (PAK1 PBD, agarose) (Cat.# 14-325)
Applications
Application Non-radioactive Rac1 Activation Assay Kit can be used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody.
Key Applications
  • Affinity Binding Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Gene Symbol
  • RAC1
  • MGC111543
  • ORFNames=MIG5
  • PAK1
  • MIG5
  • TC-25
  • p21-Rac1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P63000 # Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP- dependent manner, suggesting that the insertion does not completely abolish effector interaction.
SIZE: 192 amino acids; 21450 Da
SUBUNIT: Interacts with the GEF proteins PREX1, RASGRF2, DOCK1, DOCK2 and DOCK7, which promote the exchange between GDP and GTP, and therefore activate it. Interacts with PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Part of a quaternary complex containing PARD3, some PARD6 protein (PARD6A, PARD6B or PARD6G) and some atypical PKC protein (PRKCI or PRKCZ), which plays a central role in epithelial cell polarization. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Interacts with RALBP1 via its effector domain. Interacts with PLXNB1. Part of a complex with MAP2K3, MAP3K3, CCM2 and DEF6. Interacts with BAIAP2, CYFIP1/SRA-1 and DEF6. Interacts with Y.pseudotuberculosis YPKA and PLCB2.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (By similarity). Melanosome. Note=Inner surface of plasma membrane possibly with attachment requiring prenylation of the C- terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
TISSUE SPECIFICITY: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
DOMAIN: SwissProt: P63000 The effector region mediates interaction with DEF6.
SIMILARITY: Belongs to the small GTPase superfamily. Rho family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by precipitating Rac-GTP from 3T3 cell lysates that had been loaded with GTPγS. The precipitated Rac-GTP was detected by immunoblot analysis using anti-Rac.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 1 kit
Material Package Kit capacity: 30 assays
Transport Information
Supplemental Information
Specifications

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