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Recombinant Human IL-15

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货号：IL013

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human Human Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of IL-15.
Description
Catalogue Number IL013
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Interleukin-15 Protein, Recombinant human
Overview Interleukin-15 (IL-15) is a potent lymphoid cell growth factor that exerts its biological activities primarily on T-cells. Human IL-15 is a 12.9 kDa protein containing 114 amino acid residues.
Alternate Names
  • IL-15
References
Product Information
Presentation Protein lyophilized from 10 mM Sodium Phosphate, pH 8.5.
Applications
Application The recombinant human Interleukin-15 protein (or IL-15 protein) is a potent lymphoid cell growth factor that exerts its biological activities primarily on T-cells.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, IL-15 exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): T-cells, NK cells, LAK cells.
Biological Information
Purity Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg of IL-15.
Source Human
Specific Activity Human IL-15 is fully biologically active when compared to standards. The ED(50) as determined by the dose-dependent proliferation of CTLL-2 cells was found to be <= 0.5 ng/mL, corresponding to a specific activity of >= 2 x 10(E6) units/mg.
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.
Gene Symbol
  • IL15
  • MGC9721
  • IL-15
UniProt Number
UniProt Summary FUNCTION: SwissProt: P40933 # Cytokine that stimulates the proliferation of T- lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
SIZE: 162 amino acids; 18086 Da
SUBCELLULAR LOCATION: Isoform IL15-S48AA: Secreted. & Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
TISSUE SPECIFICITY: Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15- S21AA is preferentially expressed in tissues such as testis and thymus.
SIMILARITY: SwissProt: P40933 ## Belongs to the IL-15 family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable at room temperature, is best stored at -20°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted IL-15 should be stored in working aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 10 µg
Transport Information
Supplemental Information
Specifications

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