[关闭]
您好！欢迎来到炼石商城   请登录  注册    兼职小伙伴  
我的购物车
0
Qiagen 凯杰 Biosharp Omega

首页重组蛋白Millipore 密理博>

图片仅供参考，请以实物为准
收藏

ChemiScreen™ Membrane Prep Human Recombinant PAC1-long

市场价：
0.00
促销价：
¥0.00

货号：HTS114M

品牌：Millipore 密理博

配送至：

支付： 账期 货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

客服 郭恒 蔡玉坤 曾宪飞 技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次
RNeasy Mini Kit (50) 50次
3720.00
Plasmid Mini Kit I(200) 200次
Plasmid Mini Kit I(200) 200...
576.00
10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱
10ul吸头，透明，袋装，可灭菌...
85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10
QIAfilter Plasmid Maxi Kit (...
3000.00
QIAamp DNA Blood Midi Kit (20) 20次
QIAamp DNA Blood Midi Kit (2...
2680.00
QIAamp UCP Pathogen Mini Kit (50)
QIAamp UCP Pathogen Mini Kit...
4112.00
Replacement Information

重要规格表

Species Host Cells Protein Target
Human Chem-1 PAC1 long isoform / PACAP
Description
Catalogue Number HTS114M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
Description ChemiSCREEN™ Human PAC1-long Receptor Membrane Preparation
Overview Human ADCYAP1R1 cDNA encoding the long isoform of PAC1
Background Information PACAP (pituitary adenylyl cyclase-activating peptide) is a peptide that exists in 2 forms, 27 or 38 amino acids, and is related to vasoactive intestinal peptide (VIP). Three related class B GPCRs, PAC1, VPAC1 and VPAC2, bind to PACAP; however, VPAC1 and VPAC2 have a much higher affinity for VIP than does PAC1 (Vaudry et al., 2000). Several splice variants of PAC1 result in proteins that differ at the N-terminus and third intracellular loop; these variants differ in their affinities for PACAP and abilities to activate Gq and Gs. High expression of PAC1 is observed in the CNS and the adrenal medulla. Studies with PAC1-null mice indicate that PAC1 plays important roles in regulation of circadian rhythms, neutrophil migration, and pulmonary vascular tone (Hannibal et al., 2001; Martinez et al., 2005; Otto et al., 2004). Chemicon's PAC1-long membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PAC1-long interactions with PACAP27. The membrane preparations exhibit a Kd of 2.7 nM for [125I]-PACAP27. With 5 µg/well PAC1-long Membrane Prep and 0.75 nM [125]-PACAP27, a greater than 20-fold signal-to-background ratio was obtained.
References
Product Information
Format Membranes
Presentation Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Applications
Application Human PAC1 long isoform / PACAP GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application Notes Radioligand binding assay, and GTPγS binding.
Biological Information
Entrez Gene Number
Entrez Gene Summary This gene encodes type I adenylate cyclase activating polypeptide receptor, which is a membrane-associated protein and shares significant homology with members of the glucagon/secretin receptor family. This receptor mediates diverse biological actions of adenylate cyclase activating polypeptide 1 and is positively coupled to adenylate cyclase. Alternative splicing of two exons of this gene generates four major splice variants, but their full-length nature has not been determined.
Gene Symbol
  • ADCYAP1R1
  • PACAPRI
  • PACAP-R-1
  • PACAPR
GPCR Class B
Host Cells Chem-1
Protein Target PAC1 long isoform / PACAP
Target Sub-Family VIP/PACAP
UniProt Number
UniProt Summary FUNCTION: SwissProt: P41586 # This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
SIZE: 468 amino acids; 53314 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.
SIMILARITY: SwissProt: P41586 ## Belongs to the G-protein coupled receptor 2 family.
Incubation Conditions Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted. Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C Radioligand: [125I] PACAP27 (Perkin Elmer # NEX294) Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 20-fold signal:background with 125I-labeled PACAP27 at 0.75 nM.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information

Signal:background and specific binding values obtained in a competition binding assay with varying amounts of PAC1-long membrane prep:
Product Usage Statements
10 µg/well 5 µg/well
Signal:Background 22.9 29
Specific Binding (cpm) 63650 62987


SPECIFICATIONS:
1 unit = 5 µg membrane preparation
Bmax: 52.0 pmol/mg
Kd: 2.7 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size 200 units
Transport Information
Supplemental Information
Specifications

温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！

我要咨询

咨询内容：
购物指南 免费注册 批量采购 购物流程 购物保障
配送方式 上门自提 第三方配送 炼石配送
支付方式 货到付款 账期付款
售后服务 售后政策 退款说明 售后流程 免责条款

服务热线

0771-3293894

关于我们|帮助中心|诚聘英才|炼石云实验室|联系我们|友情链接

Copyright 2014-2025 炼石云商网络科技（海南）有限公司版权所有 琼ICP备2023006525号-3