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RalB Activation Assay Kit (non-radioactive)

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货号：17-439

品牌：Millipore 密理博

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Replacement Information

重要规格表

Species Reactivity Key Applications
H, M, R GPA
Description
Catalogue Number 17-439
Brand Family Upstate
Trade Name
  • Upstate
Description RalB Activation Assay Kit (non-radioactive)
Alternate Names
  • RAS-like protein B
  • v-ral simian leukemia viral oncogene homolog B
  • GTP binding protein
Materials Required but Not Delivered Stimulated and Non-stimulated Cell Lysates
Pipettes, Micropipettes and Tips
Electrophoresis and Immunoblotting Equipment
Microcentrifuge
Microcentrifuge Tubes
Ral Activators
Tissue Culture Supplies
4C Tube Rocker
Glycerol
aprotinin
PMSF
Anti-Mouse Secondary Antibody and Detection Reagents
TRIS Buffered Saline (TBS), Ice Cold
References
Product Information
Components
  • anti-RalB, clone 25 (Cat.# 04-037)
  • Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
  • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
  • 100X GTPγS, 10mM (Cat.# 20-176)
  • 100X GDP, 100mM (Cat.# 20-177)
Applications
Application Non-radioactive Ral-B Activation Assay Kit can be used to precipitate Ral-B-GTP from cell lysates & detection by a Ral-B specific monoclonal antibody.
Key Applications
  • G-Protein Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Entrez Gene Number
Gene Symbol
  • RALBP1
  • RLIP1
  • RIP1
  • RalBP1
  • RIP
  • RLIP76
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q15311 # Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDC2 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
SIZE: 655 amino acids; 76063 Da
SUBUNIT: Interacts with the GTP-bound form of RALA, RALB, CDC42 and RAC1. Interacts with REPS1 and REPS2 and this does not affect the Ral-binding activity. Interacts with DAB2IP. Interacts with catalytically active CCNB1 and CDC2 during mitosis. Interacts with EPN1, NUMB and TFAP2A during interphase and mitosis.
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Expressed ubiquitously but at low levels. Shows a strong expression in the erythrocytes.
SIMILARITY: SwissProt: Q15311 ## Contains 1 Rho-GAP domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by non-radioactive Kinase Assay.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C from date of shipment
Packaging Information
Material Size 20 assays
Transport Information
Supplemental Information
Specifications

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