VEGF165 , Human recombinant

Key Applications	Species	Source	Purity
CULT	Human	E.coli	Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF

Key Applications

Species

Source

Purity

CULT

Human

E.coli

Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF

Description
Catalogue Number	GF094
Replaces	GF025
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform
Overview	Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.
Alternate Names	VEGF

Description

Catalogue Number

GF094

Replaces

GF025

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform

Overview

Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.

Alternate Names

VEGF

Product Information
Presentation	Lyophilized from sterile filtered 20 mM acetic acid, containing no additives.

Product Information

Presentation

Lyophilized from sterile filtered 20 mM acetic acid, containing no additives.

Applications
Application	Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues.
Key Applications	Cell Culture
Application Notes	For most in vitro applications, human VEGF exerts its biological activity in the concentration range of 0.1 to 50.0 ng/mL. Responding cells are (partial list): Endothelial cells.

Applications

Application

Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues.

Key Applications

Cell Culture

Application Notes

For most in vitro applications, human VEGF exerts its biological activity in the concentration range of 0.1 to 50.0 ng/mL. Responding cells are (partial list): Endothelial cells.

Biological Information
Purity	Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF
Source	E.coli
Specific Activity	The biological activity of Millipore's human VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC). Mitogenic activity was obtained in a concentration range of 1.0-8.0 ng/mL.
Entrez Gene Number	NM_001025366.1 NM_001025367.1 NM_001025368.1 NM_001025369.1 NM_001025370.1 NM_001033756.1 NM_003376.4
Entrez Gene Summary	This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Gene Symbol	VEGFA MGC70609 VPF VEGF VEGF-A
UniProt Number	P15692
UniProt Summary	FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SIZE: 232 amino acids; 27042 Da SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon. SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.
Stem Cell Type	Neural Stem Cells Mesenchymal Stem Cells Hematopoietic Stem Cells
Molecular Weight	38.2 kDa

Biological Information

Purity

Greater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1 EU/ug) of VEGF

Source

E.coli

Specific Activity

The biological activity of Millipore's human VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC). Mitogenic activity was obtained in a concentration range of 1.0-8.0 ng/mL.

Entrez Gene Number

Entrez Gene Summary

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Gene Symbol

VEGFA
MGC70609
VPF
VEGF
VEGF-A

UniProt Number

P15692

UniProt Summary

FUNCTION: SwissProt: P15692 # Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SIZE: 232 amino acids; 27042 Da
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
TISSUE SPECIFICITY: The VEGF189, VEGF-165 and VEGF-121 isoforms are widely expressed, whereas the VEGF206 and VEGF-145 are uncommon.
SIMILARITY: SwissProt: P15692 ## Belongs to the PDGF/VEGF growth factor family.

Stem Cell Type

Neural Stem Cells
Mesenchymal Stem Cells
Hematopoietic Stem Cells

Molecular Weight

38.2 kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. Reconstituted material can be stored at 2°C-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to one year from date of receipt. After a quick spin, reconstitute with sterile water to a concentration of 0.1-1.0 mg/mL. Reconstituted material can be stored at 2°C-8°C for one week, but undiluted aliquots at -20°C are recommended. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	10 µg

Packaging Information

Material Size

10 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

VEGF165 , Human recombinant

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