Human RBP4 Detection Antibody (ELISA) (6ml) - 炼石商城

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Human RBP4 Detection Antibody (ELISA) (6ml)

市场价：: 0.00

促销价：: ¥0.00

货号：E1018

品牌：Millipore 密理博

配送至：

北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Species Reactivity	Host	Antibody Type
Ca, H, Mk, M, Rb, R	M	Monoclonal Antibody

价格及供货情况

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Description
Overview	This product has been discontinued. Recognizes the ~21 kDa myelin basic protein in rat brain tissue.
Catalogue Number	NE1018
Brand Family	Calbiochem®
Application Data	Detection of rat myelin basic protein by staining frozen sections. Sample: Rat brain. Primary antibody: Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) (1:1000). Detection: fluorescence (green) with Hoechst counterstain.

References
References	Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods 72, 197. Shin, R.W., et al. 1991. Lab. Invest. 64, 693.

Product Information
Form	Liquid
Formulation	Undiluted ascites.
Positive control	Rat brain
Preservative	≤0.1% sodium azide

Applications
Key Applications	Immunocytochemistry Paraffin Sections Enzyme-Linked Immunosorbent Assay Frozen Sections Immunoblotting (Western Blotting)
Application Notes	ELISA (1:1000) Frozen Sections (1:1000, see comments) Immunoblotting (1:1000) Immunocytochemistry (1:1000) Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Application Comments	Other species have not been tested, but the antibody is expected to recognize MBP in most mammalian species. Recognizes developing and adult myelin and developing oligodendrocytes. Distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue. In conjunction with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-99) (Cat. No. NE1019), this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Tissues can be fixed in a variety of paraformaldehyde- or formaldehyde-containing fixatives. To insure penetration of the antibody into myelin it is necessary to pre-treat frozen sections with cold methanol for 30 min or detergent. For staining paraffin sections it is recommended that de-paraffinized tissues be autoclaved in dH₂O for 10 min or boiled in Tris-saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	purified human myelin basic protein
Immunogen	Human
Epitope	Within amino acids 70-89
Clone	SMI-94
Host	Mouse
Isotype	IgG₁
Species Reactivity	Canine Human Monkey Mouse Rabbit Rat
Antibody Type	Monoclonal Antibody

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Dry Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

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