Recombinant Human FGF-8

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Recombinant Human FGF-8

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货号：GF110

品牌：Millipore 密理博

配送至：

北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Key Applications	Species	Source	Purity
CULT	Human	E.coli	Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of FGF-8.

Description
Catalogue Number	GF110
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Fibroblast Growth Factor-8 Protein, Recombinant human
Overview	Fibroblast Growth Factor-8 (FGF-8) is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. Recombinant Human FGF-8 is a 22.4 kDa protein containing 193 amino residues.
Alternate Names	FGF-8

References

Product Information
Presentation	The sterile filtered solution was lyophilized from 5 mM NaP, pH 7.5 + 5mM NaCl.

Applications
Application	The Fibroblast Growth Factor-8 (or FGF-8 protein) is a heparin binding growth factor that stimulates the proliferation & activation of cells that express FGF receptors.
Key Applications	Cell Culture

Biological Information
Purity	Greater than 95% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of FGF-8.
Source	E.coli
Specific Activity	The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is <=0.5 ng/ml, corresponding to a specific activity of >= 1 x 10(E6) units/mg. The protein may appear as a haze or film, which you may not see at the bottom of the vial.
Entrez Gene Number	NM_006119.2 NM_033163.1 NM_033164.1 NM_033165.1
Entrez Gene Summary	The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants.
Gene Symbol	FGF8 MGC149376 HBGF-8 FGF-8 AIGF
UniProt Number	P55075
UniProt Summary	FUNCTION: SwissProt: P55075 # Stimulates growth of the cells in an autocrine manner. Mediates hormonal action on the growth of cancer cells. SIZE: 233 amino acids; 26525 Da SUBCELLULAR LOCATION: Secreted. DEVELOPMENTAL STAGE: In adults expression is restricted to the gonads. SIMILARITY: SwissProt: P55075 ## Belongs to the heparin-binding growth factors family.
Stem Cell Type	Neural Stem Cells Epithelial Cells Mesenchymal Stem Cells Pancreatic Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to 12 months after date of receipt. After a quick spin, reconstitute with 10 mM Tris, pH 8.0 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted human FGF-8 should be stored in undiluted aliquots at -20ºC for upt to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C for up to 12 months after date of receipt. After a quick spin, reconstitute with 10 mM Tris, pH 8.0 to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted human FGF-8 should be stored in undiluted aliquots at -20ºC for upt to 6 months. Avoid repeated freeze/thaw cycles.