Recombinant Human CNTF

Species	Source	Purity
Human	E.coli	Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of CNTF.

Species

Source

Purity

Human

E.coli

Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of CNTF.

Description
Catalogue Number	GF109
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Ciliary Neurotrophic Factor Protein, Recombinant human
Overview	Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF also has been shown to promote survivability and differentiation of other neural cell types. Recombinant Human CNTF is a 22.7 kDa protein containing 199 amino acid residues.
Alternate Names	CNTF

Description

Catalogue Number

GF109

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Ciliary Neurotrophic Factor Protein, Recombinant human

Overview

Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. CNTF also has been shown to promote survivability and differentiation of other neural cell types. Recombinant Human CNTF is a 22.7 kDa protein containing 199 amino acid residues.

Alternate Names

CNTF

Product Information
Presentation	The sterile filtered solution was lyophilized from 10 mM Citric Acid, pH 3.0 + 75 mM sodium chloride.

Product Information

Presentation

The sterile filtered solution was lyophilized from 10 mM Citric Acid, pH 3.0 + 75 mM sodium chloride.

Applications
Application	The human Ciliary Neurotrophic Factor (or CNTF protein) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro.

Applications

Application

The human Ciliary Neurotrophic Factor (or CNTF protein) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro.

Biological Information
Purity	Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of CNTF.
Source	E.coli
Specific Activity	The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150 ng/mL.
Entrez Gene Number	NM_000614.2
Entrez Gene Summary	The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.
Gene Symbol	CNTF HCNTF
UniProt Number	P26441
UniProt Summary	FUNCTION: SwissProt: P26441 # CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SIZE: 200 amino acids; 22931 Da SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. SIMILARITY: SwissProt: P26441 ## Belongs to the CNTF family.

Biological Information

Purity

Greater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of CNTF.

Source

E.coli

Specific Activity

The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is 50-150 ng/mL.

Entrez Gene Number

NM_000614.2

Entrez Gene Summary

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.

Gene Symbol

CNTF
HCNTF

UniProt Number

P26441

UniProt Summary

FUNCTION: SwissProt: P26441 # CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
SIZE: 200 amino acids; 22931 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Nervous system.
SIMILARITY: SwissProt: P26441 ## Belongs to the CNTF family.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C. Reconstitute in 5 mM NaP, pH 7.5 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted human CNTF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

The lyophilized powder is stable for a few weeks at room temperature, but best stored at -20°C. Reconstitute in 5 mM NaP, pH 7.5 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted human CNTF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	20 µg

Packaging Information

Material Size

20 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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