Recombinant Human CD40 LIGAND / TRAP

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Recombinant Human CD40 LIGAND / TRAP

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货号：GF101

品牌：Millipore 密理博

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北京朝阳

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EA (预计3-5工作日到货)

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Replacement Information

重要规格表

Key Applications	Species	Source	Purity
CULT	Human	Recombinant human	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.

Description
Catalogue Number	GF101
Brand Family	Chemicon®
Trade Name	Chemicon
Description	CD40 Ligand/TRAP Protein, Recombinant human
Overview	CD40 Ligand (CD40L/TRAP) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells. GF101 is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Alternate Names	CD154 TRAP TNF-related activation protein T cell antigen Gp39

References

Product Information
Presentation	Sterile filtered and lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Applications
Application	The CD40 Ligand (CD40L/TRAP protein) is a type II membrane protein which, effectuates the helper function of T cells on resting B cells.
Key Applications	Cell Culture

Biological Information
Purity	Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg) of sCD40L/TRAP.
Source	Recombinant human
Specific Activity	GF101 is fully biologically active as determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected ED50 for this effect is 5-10 ng/ml. Note: Results may vary with PBMC donors.
Entrez Gene Number	NM_000074.2
Entrez Gene Summary	The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Gene Symbol	CD40LG CD154 TRAP gp39 CD40L IMD3 IGM hCD40L TNFSF5 HIGM1 CD40-L T-BAM
UniProt Number	P29965
UniProt Summary	FUNCTION: SwissProt: P29965 # Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. SIZE: 261 amino acids; 29274 Da SUBUNIT: Homotrimer. SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & CD40 ligand, soluble form: Secreted. TISSUE SPECIFICITY: Specifically expressed on activated CD4+ T- lymphocytes. PTM: The soluble form derives from the membrane form by proteolytic processing. & N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40. & Not O-glycosylated. DISEASE: SwissProt: P29965 # Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. SIMILARITY: SwissProt: P29965 ## Belongs to the tumor necrosis factor family.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in 10 mM Sodium Phosphate, pH7.5 to a concentration of 0.1-1 mg/mL. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.

Storage and Shipping Information

Storage Conditions

The lyophilized protein is stable for a few weeks for at room temperature but is best stored at -20ºC. Reconstitute in 10 mM Sodium Phosphate, pH7.5 to a concentration of 0.1-1 mg/mL. This solution can be diluted into other aqueous buffers and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted sCD40L/TRAP should be stored at -20ºC in undiluted aliquots for up to 6 months. Freeze/thaw cycles will result in significant loss of activity.