Recognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
Catalogue Number
GF06
Brand Family
Calbiochem®
Synonyms
Anti-Transforming Growth Factor-α
References
References
Sorvillo, J.M., McCormack, E.S. 1990. Oncogene5, 377. Coffey, R.J., et al. 1987. Nature328, 817. Derynck, R. 1986. J. Cell. Biochem.32, 203. Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408. Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644. Kimball, E.S., et al. 1984. Cancer Res.44, 3613. Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684. Reynolds, F.H., Jr., et al. 1983. Nature292, 259. Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
Product Information
Form
Liquid
Formulation
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative control
NIH3T3 cells
Positive control
A2058 or ST-FeSV FRE cells
Preservative
≤0.1% sodium azide
Applications
Application References
Original Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis22, 1809. Bush, M.R., et al. 1998. Biol. Reprod.59, 1522.
Key Applications
Immunoblotting (Western Blotting)
Application Notes
Immunoblotting (1-5 µg/ml, see application references)
Application Comments
This antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
recombinant, human TGF-α (~6 kDa mature form)
Immunogen
Human
Clone
134A-2B3
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Rat
Antibody Type
Monoclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Yes
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
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