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Recombinant Rat CNTF

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货号：GF035

品牌：Millipore 密理博

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Replacement Information

重要规格表

Species Source Purity
Rat E.coli Greater than 98% by SDS-PAGE and HPLC analysis
Description
Catalogue Number GF035
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Ciliary Neurotrophic Factor Protein, Recombinant rat
Overview Ciliary Neurotrophic Factor (CNTF) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro. More recently, CNTF has been shown to promote survivability and differentiation of other neuronal cell types. Rat CNTF is a 22.7 kDa protein containing 199 amino acid residues.
Alternate Names
  • CNTF
References
Product Information
Presentation Sterile filtered, lyophilized from 5 mM Na Acetate, pH 6.5. Contains no preservative.
Applications
Application The Rat Ciliary Neurotrophic Factor (or CNTF protein) is a potent neural factor that was originally characterized as a survivability factor for chick ciliary neurons in vitro.
Application Notes Concentration range for most in vitro applications is 0.01-10 ng/mL. Responding cells are (partial list): Ciliary and sympathetic neurons.
Biological Information
Purity Greater than 98% by SDS-PAGE and HPLC analysis
Source E.coli
Specific Activity The rat CNTF is fully biologically active when compared to standards. The ED(50) as determined by the dose-dependent proliferation of human TF-1 cells is <= 15-30 ng/mL.
Entrez Gene Summary The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.
UniProt Number
UniProt Summary FUNCTION: SwissProt: P26441 # CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
SIZE: 200 amino acids; 22931 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Nervous system.
SIMILARITY: SwissProt: P26441 ## Belongs to the CNTF family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute with sterile water to a concentration of 100 mg/mL. This solution can be diluted into water or other buffered solutions. Reconstituted GF035 should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size 25 µg
Transport Information
Supplemental Information
Specifications

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