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p53, GST Recombinant Protein

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货号：14-865

品牌：Millipore 密理博

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Description
Catalogue Number 14-865
Brand Family Upstate
Trade Name
  • Upstate
Description p53-GST (recombinant protein)
Overview N-terminal GST-tagged, recombinant full length human P53 protein, expressed in E. coli. Purified using glutathione agarose chromatography.
Alternate Names
  • transformation related protein 53
References
Product Information
Presentation Recombinant protein in buffer containing 20 mM Tris-HCl, pH 8.0, 100 mM KCl, 5mM glutathione, 0.5 mM EDTA, 0.5 mM DTT, 1 mM PMSF containing 20% glycerol.
Applications
Key Applications
  • Enzyme Assays
Biological Information
Concentration 0.5 mg/mL
Purity ~95% by SDS-PAGE
Source Full-length human p53 protein, expressed in e. coli.
Specific Activity For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene Summary This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]
Gene Symbol
  • p53
  • TP53
  • tumor protein p53
  • TRP53
  • FLJ92943
  • LFS1
Purification Method Glutathione agarose chromatography
UniProt Number
Molecular Weight 72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Routinely evaluated by SDS-PAGE and Coomassie Stain
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions 1 year at -20°C
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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