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Rat/Mouse Ghrelin (Total) ELISA Kits COLD PACKS

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货号：EZRGRT-91K

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Ghrelin (Total) R, M ELISA Fluorescent, Colorimetric
Description
Catalogue Number EZRGRT-91K
Description Rat/Mouse Ghrelin (total) ELISA
Background Information Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.
References
Product Information
Detection method Fluorescent Colorimetric
Precision, %
  • Intra-assay: Serum: 0.7–1.3% Plasma: 1.1–1.7%
  • Inter-assay: Serum: 1.8-4.5% Plasma: 2.0-3.2%
Applications
Application This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application Notes Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)
Biological Information
Specificity Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%
Species Reactivity
  • Rat
  • Mouse
Analytes Available
  • Ghrelin (Total)
UniProt Number
Accuracy
  • Plasma: (Active Ghrelin) 86-102%
  • Plasma: (Des-octanoyl Ghrelin) 95-107%
  • Serum: (Des-octanoyl Ghrelin) 92-103%
  • Serum: (Active Ghrelin) 80-103%
Physicochemical Information
Linearity of Dilution Contact Technical Service for this information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size 96-well strip plate
Transport Information
Supplemental Information
Specifications

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