Recombinant Rat LIF

Key Applications	Species	Source	Purity
Cult, CULT	Rat	rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.	Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests.

Key Applications

Species

Source

Purity

Cult, CULT

Rat

rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests.

Description
Catalogue Number	LIF3010
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Leukemia Inhibitory Factor Protein, Recombinant rat
Overview	Rat LIF (rtLIF) is a 22.1 kDa protein containing 202 amino acid residues that exhibits 91% amino acid sequence identity with murine LIF(Takahama etal 1998).Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama, et al 1998).
Alternate Names	LIF
Background Information	Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

Description

Catalogue Number

LIF3010

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Leukemia Inhibitory Factor Protein, Recombinant rat

Overview

Rat LIF (rtLIF) is a 22.1 kDa protein containing 202 amino acid residues that exhibits 91% amino acid sequence identity with murine LIF(Takahama etal 1998).Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama, et al 1998).

Alternate Names

Background Information

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo.

Product Information
Presentation	Liquid in 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl, pH 7.4 and 0.02% Tween 20. No preservativies added.

Product Information

Presentation

Liquid in 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl, pH 7.4 and 0.02% Tween 20. No preservativies added.

Applications
Application	Recombinant rat Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.
Key Applications	Stem Cell Culture Cell Culture
Application Notes	RECOMMENDED QC PROTOCOL M1 Bioassay 1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar. 2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes. 3.Add 100 μL of 5% FCS in isotonic saline to two control slides. 4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days. 5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated). Visit www.esgro-lif.com for additional information

Applications

Application

Recombinant rat Leukemia Inhibitory Factor (LIF) protein is biologically active and suitable for cell culture applications.

Key Applications

Stem Cell Culture
Cell Culture

Application Notes

RECOMMENDED QC PROTOCOL

M1 Bioassay

1.The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2.Add 100 μL of sample or rtLIF (10(e4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3.Add 100 μL of 5% FCS in isotonic saline to two control slides.

4.Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5.Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).

Visit www.esgro-lif.com for additional information

Biological Information
Concentration	10 μg/mL
Purity	Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests.
Source	rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.
Specific Activity	The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(e8) units/mg, where 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.
Entrez Gene Summary	Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
Gene Symbol	NM_022196
UniProt Number	P17777
UniProt Summary	FUNCTION: SwissProt: P17777 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22113 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P17777 ## Belongs to the LIF/OSM family.
Stem Cell Type	Mouse Embryonic Stem Cells Neural Stem Cells Hematopoietic Stem Cells Mesenchymal Stem Cells Induced Pluripotent Stem Cells

Biological Information

Concentration

10 μg/mL

Purity

Greater than 85% by analytical HPLC and SDS-PAGE. Endotoxin level is less than 0.1 ng per μg of LIF. Tested negative in both aseptic and mycoplasma tests.

Source

rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.

Specific Activity

The activity of rat LIF is determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5 ng/mL. The specific activity is >=1 x 10(e8) units/mg, where 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1 mL agar cultures.

Entrez Gene Summary

Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.

Gene Symbol

NM_022196

UniProt Number

P17777

UniProt Summary

FUNCTION: SwissProt: P17777 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
SIZE: 202 amino acids; 22113 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P17777 ## Belongs to the LIF/OSM family.

Stem Cell Type

Mouse Embryonic Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Mesenchymal Stem Cells
Induced Pluripotent Stem Cells

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Rat LIF is shipped on dry ice. Maintain at -20°C until expiration date. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added and stored at -20°C. Freeze thawing is not recommended.

Storage and Shipping Information

Storage Conditions

Rat LIF is shipped on dry ice. Maintain at -20°C until expiration date. Further dilutions should be made into buffer or medium to which protein (e.g., 1% BSA) or Tween 20 has been added and stored at -20°C. Freeze thawing is not recommended.

Packaging Information
Material Size	10 µg

Packaging Information

Material Size

10 µg

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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