Human RBP4 ELISA Microtiter Strip Plate.

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, ICC	Rb	Unpurified	Monoclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

WB, ICC

Unpurified

Monoclonal Antibody

Description
Catalogue Number	04-1009
Description	Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal
Alternate Names	acetyl-CoA carboxylase 1 acetyl-CoA carboxylase-alpha acetyl-Coenzyme A carboxylase alpha
Background Information	Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Description

Catalogue Number

04-1009

Description

Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal

Alternate Names

acetyl-CoA carboxylase 1
acetyl-CoA carboxylase-alpha
acetyl-Coenzyme A carboxylase alpha

Background Information

Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

Product Information
Format	Unpurified
Control	Untreated and LP-treated A431 cell lysate
Presentation	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Product Information

Format

Unpurified

Control

Untreated and LP-treated A431 cell lysate

Presentation

Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Applications
Application	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

Applications

Application

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

Key Applications

Western Blotting
Immunocytochemistry

Application Notes

Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

Biological Information
Immunogen	Phospho-specific peptide corresponding to residues surrounding Ser79 of human ACC1.
Epitope	Phosphorylated Ser79
Clone	EP1885Y
Host	Rabbit
Specificity	This antibody detects ACC1 phosphorylated at Ser79.
Isotype	IgG
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_942131.1
Entrez Gene Summary	Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
Gene Symbol	ACAC ACC ACC-alpha ACC1 ACCA EC 6.4.1.2 OTTHUMP00000164069
Modifications	Phosphorylation Acetylation
Purification Method	Unpurified
UniProt Number	Q13085
UniProt Summary	FUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA. ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein. COFACTOR: Biotin. Binds 2 manganese ions per subunit. ENZYME REGULATION: By phosphorylation By similarity. PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1. INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth. SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. Contains 1 carboxyltransferase domain.
Molecular Weight	~280 kDa

Biological Information

Immunogen

Phospho-specific peptide corresponding to residues surrounding Ser79 of human ACC1.

Epitope

Phosphorylated Ser79

Clone

EP1885Y

Host

Rabbit

Specificity

This antibody detects ACC1 phosphorylated at Ser79.

Isotype

IgG

Species Reactivity

Human

Antibody Type

Monoclonal Antibody

Entrez Gene Number

NP_942131.1

Entrez Gene Summary

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Gene Symbol

ACAC
ACC
ACC-alpha
ACC1
ACCA
EC 6.4.1.2
OTTHUMP00000164069

Modifications

Phosphorylation
Acetylation

Purification Method

Unpurified

UniProt Number

Q13085

UniProt Summary

FUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.

ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein.

COFACTOR: Biotin.

Binds 2 manganese ions per subunit.

ENZYME REGULATION: By phosphorylation By similarity.

PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1.

INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth.

SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Contains 1 carboxyltransferase domain.

Molecular Weight

~280 kDa

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in untreated and LP-treated A431 cell lysates. Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect ACC1 in untreated and LP-treated A431 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Quality Assurance

Evaluated by Western Blot in untreated and LP-treated A431 cell lysates.
Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect ACC1 in untreated and LP-treated A431 cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Storage and Shipping Information

Storage Conditions

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Human RBP4 ELISA Microtiter Strip Plate.

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