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Recombinant Human IGF-II

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货号：GF007

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E.coli Greater than 98% by SDS-PAGE and HPLC analysis
Description
Catalogue Number GF007
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Insulin-like Growth Factor-II Protein, Recombinant human
Overview Insulin Like Growth Factor-II (IGF-II) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types. Human IGF-II is a 7.5 kDa protein containing 67 amino acid residues.
Alternate Names
  • IGF-II
References
Product Information
Presentation Lyophilized powder with no additives
Applications
Application The Insulin Like Growth Factor-II protein (or IGF-2 protein) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, IGF-II exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Bone, muscle cells.

Optimal working dilutions must be determined by end user.

Optimal working dilutions must be determined by end user.
Biological Information
Purity Greater than 98% by SDS-PAGE and HPLC analysis
Source E.coli
Specific Activity Chemicon's Human IGF-II is fully biologically active when compared to standards. The ED50 as determined by a cell proliferation assay using FDC-P1 cells is <= 2.0 ng/mL, corresponding to a specific activity of >= 5 x 105 units/mg.
Entrez Gene Number
Entrez Gene Summary This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • IGF2
  • FLJ44734
  • pp9974
  • ORFNames=PP1446
  • C11orf43
  • IGF-II
  • INSIGF
  • Somatomedin-A
  • FLJ22066
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
SIZE: 180 amino acids; 20140 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family.
Stem Cell Type
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized IGF-II, though stable at room temperature, is best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted IGF-II should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 50 µg
Transport Information
Supplemental Information
Specifications

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