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Qiagen 凯杰 Biosharp Omega

首页细胞生物学检测Millipore 密理博>

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Human Insulin Specific RIA 250TubeKit I-125<185KBqs

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货号：HI-14K

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Insulin H ELISA Fluorescent, Colorimetric
Description
Catalogue Number EZHI-14K
Description Human Insulin ELISA
Background Information Insulin, a 5.8 kDa hormone, is secreted by the islet beta cells in the pancreas.
References
Product Information
Detection method Fluorescent Colorimetric
Precision, %
  • Inter-assay: 9.1–11.4
  • Intra-assay: 4.6–7.0
Applications
Application This Human Insulin ELISA is used to measure & quantify insulin levels in Metabolism & Endocrine research.
Key Applications
  • ELISA
Application Notes 2 hour assay, room temperature Sample ≤ 20 μL serum, plasma, or tissue culture medium
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Insulin
Entrez Gene Number
Entrez Gene Summary After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
Gene Symbol
  • INS
  • proinsulin
  • insulin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
SIZE: 110 amino acids; 11981 Da
SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
Accuracy
  • 76–103%
Physicochemical Information
Linearity of Dilution 96–112%
Standard Curve Range
  • 2–200 μU/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size 96-well strip plate
Transport Information
Supplemental Information
Specifications

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