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Qiagen 凯杰 Biosharp Omega

首页ELISA试剂盒Millipore 密理博>

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Human Leptin ELISA kit

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货号：EZHL-80SK

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Leptin H ELISA Fluorescent, Colorimetric
Description
Catalogue Number EZHL-80SK
Description Human Leptin "Dual Range" ELISA
Background Information Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
References
Product Information
Detection method Fluorescent Colorimetric
Precision, %
  • Inter-assay: 2.6–6.2 Sensitive Assay 1.3–8.6
  • Intra-assay: 2.6–4.6 Sensitive Assay 1.4–4.9
Applications
Application This Human Leptin "Dual Range" ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.
Key Applications
  • ELISA
Application Notes Room temperature, 3.5 hour assay 25 μL sample size (Sensitive Assay: 50μL)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • Leptin
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
Gene Symbol
  • LEP
  • leptin
  • OB
  • obesity
  • OBS
UniProt Number
UniProt Summary FUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
SIZE: 167 amino acids; 18641 Da
SUBUNIT: Interacts with SIGLEC6.
SUBCELLULAR LOCATION: Secreted.
DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
Accuracy
  • 94–114%
  • Sensitive Assay 86–102%
Physicochemical Information
Sensitivity
  • 0.5 ng/mL
  • Sensitive Assay 0.125 ng/mL
Linearity of Dilution 80–105% Sensitive Assay 96–125%
Standard Curve Range
  • 0.5–100 ng/mL
  • Sensitive Assay 0.125–20 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Packaging Information
Material Size 96-well strip plate
Transport Information
Supplemental Information
Specifications

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