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Recombinant Human Interferon gamma

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号:IF002

牌:Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human Human, expressed in E.coli Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
Description
Catalogue Number IF002
Replaces IF3
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Interferon-γ Protein, Recombinant human
Overview Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
References
Product Information
Presentation Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Applications
Application The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Biological Information
Purity Greater than 98% by SDS-PAGE and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
Source Human, expressed in E.coli
Specific Activity Human IFN-gamma is fully biologically active when compared to standards. The ED(50) as determined in a viral resistance assay is <= 0.05 ng/mL corresponding to a specific activity of >= 2 x 10(E7) units/mg.
Entrez Gene Number
Gene Symbol
  • IFNG
  • IFN-gamma
  • IFI
  • IFG
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01579 # Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
SIZE: 166 amino acids; 19348 Da
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
PTM: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
DISEASE: SwissProt: P01579 # In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
SIMILARITY: SwissProt: P01579 ## Belongs to the type II (or gamma) interferon family.
Stem Cell Type
  • Mesenchymal Stem Cells
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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