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ChemiScreen™ Membrane Prep Human Recombinant NMU1

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货号：HTS062M

品牌：Millipore 密理博

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Replacement Information

重要规格表

Species Host Cells Protein Target
Human Chem-1 NMU1
Description
Catalogue Number HTS062M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
Description ChemiScreen™ Recombinant Human NMU1 Neuromedin Receptor
Overview Human NMU1
Background Information Neuromedin U (NmU) is a peptide characterized by its ability to promote smooth muscle contraction (Brighton et al., 2004a). Two GPCRs, NMU1 and NMU2, mediate the contractile effects of neuromedin U by activation of both Gq and Gi (Brighton et al., 2004b). In addition, NmU binds to NMU1 expressed on Th2 cells to induce cytokine release (Johnson et al., 2004). Chemicon's NMU1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NMU1 interactions with neuromedin U25. The membrane preparations exhibit a Kd of 0.08 nM for [125I]-neuromedin U25. With 5 µg/well NMU1 Membrane Prep and 0.5 nM [125I]-neuromedin U25, a greater than 30-fold signal-to-background ratio was obtained.
References
Product Information
Format Membranes
Presentation One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 30-fold signal:background with 125I-labeled neuromedin U25 at 0.5 nM.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
Applications
Application Human NMU1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Key Applications
  • Radioligand binding assay
  • GTPγS binding
Application Notes Radioligand binding assay, and GTPγS binding.
Biological Information
Entrez Gene Number
Gene Symbol
  • NMUR1
  • FM3
  • (FM-3)
  • FM-3
  • GPR66
  • GPC-R
  • NMU1R
  • NMU-R1
GPCR Class A
Host Cells Chem-1
Protein Target NMU1
Target Sub-Family Neuromedin U
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q9HB89 # Receptor for the neuromedin-U and neuromedin-S neuropeptides (By similarity).
SIZE: 426 amino acids; 47351 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Expressed in greatest abundance in peripheral organs, particularly in elements of the gastrointestinal and urogenital systems with highest levels in testes. In central nervous system structures express levels are much lower than those seen in peripheral organs. Within the CNS, has been detected in highest abundance in the cerebellum, dorsal root ganglia, hippocampus, and spinal cord.
SIMILARITY: SwissProt: Q9HB89 ## Belongs to the G-protein coupled receptor 1 family.
Incubation Conditions Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-neuromedin U25 (Perkin Elmer # NEX383)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information


Signal:background and specific binding values obtained in a competition binding assay with NMU1 membrane prep.
Product Usage Statements
10 µg/well 5 µg/well
Signal:Background 15.7 20.5
Specific Binding (cpm) 4779 4829


SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax: 1.06 pmol/mg
Kd: 0.08 nM Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size 2 units
Transport Information
Supplemental Information
Specifications

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