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Recombinant Human TGF-alpha

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货号：GF022

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E.coli Greater than 98% by SDS-PAGE and HPLC analyses
Description
Catalogue Number GF022
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Transforming Growth Factor-α Protein, Recombinant human
Overview Transforming Growth Factor-a (TGF-a) is a polypeptide growth factor which stimulates the proliferation of a wide range or epidermal and epithelial cells. TGF-a has been shown a play a role in the autocrine growth of certain transformed cells. Human TGF-a is a 5.5 kDa protein containing 50 amino acid residues.
Alternate Names
  • TGF-alpha
References
Product Information
Presentation Lyophilized powder with no additives.
Applications
Application Transforming Growth Factor-a (TGF-a) is a polypeptide growth factor which stimulates the proliferation of a wide range or epidermal & epithelial cells.
Key Applications
  • Cell Culture
Application Notes For most in vitro applications, TGFalpha exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal, mesothelial cells.
Biological Information
Purity Greater than 98% by SDS-PAGE and HPLC analyses
Source E.coli
Specific Activity Chemicon's human TGF-alpha, is fully biologically active when compared to standards. The ED(50) as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <=0.2 ng/mL, corresponding to a specific activity of >=5 x 10(E6) units/mg.
Entrez Gene Number
Entrez Gene Summary Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM]
Gene Symbol
  • TGFA
  • TFGA
UniProt Number
UniProt Summary FUNCTION: SwissProt: P01135 # TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
SIZE: 160 amino acids; 17006 Da
SUBUNIT: Interacts with the PDZ domains of SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface.
SUBCELLULAR LOCATION: Transforming growth factor alpha: Secreted, extracellular space. & Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P01135 ## Contains 1 EGF-like domain.
Stem Cell Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized TGF-alpha, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted TGF-alpha should be stored at 4°C for up to one week or at -20°C in undiluted aliquots for up to six months.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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