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Qiagen 凯杰 Biosharp Omega

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ChemiScreen™ Membrane Prep Hu Recombinant C5aR

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货号：HTS017M

品牌：Millipore 密理博

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Replacement Information

重要规格表

Species Host Cells Protein Target
Human Chem-1 C5aR
Description
Catalogue Number HTS017M
Brand Family Chemicon®
Trade Name
  • ChemiScreen
  • Chemicon
Description ChemiSCREEN™ C5aR Membrane Preparation, recombinant
Overview Full-length human C5aR cDNA
Background Information C5a is a proinflammatory peptide generated through activation of the complement system, and is more potent than the other anaphylatoxins, C4a and C3a, in activating peripheral blood leukocytes (Gerard & Gerard, 1994). It can actively participate in the regulation of local cytokine network by stimulating production of proinflammatory cytokines (Buchner et al., 1995; Hsu, et al., 1999). The proinflammatory effects of C5a are mediated through binding to a specific 7-TM chemoattractant receptor, C5aR (CD88). Binding of C5a to phagocyte C5aR induces chemotaxis, production of superoxide anions, and release of degradative enzymes. Pharmacologic or genetic disruption of C5a/C5aR interaction reduces sepsis (Riedemann et al., 2003), immune complex-induced lung disease (Shushakova et al., 2002), and Arthrogen-induced arthritis (Grant et al., 2002) in experimental models. Chemicon's cloned human C5a receptor membrane preparations are ideal tools for screening for antagonists of C5a/C5aR interactions. The C5aR Membrane Preps bind specifically to 125I-labeled ligand C5a, with Kd of 0.59 nM. With 5 μg membrane per well and 0.1 nM 125I-labeled C5a, more than 15-fold signal:background was obtained.
References
Product Information
Format Membranes
Presentation Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 1000 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of I-125 labeled C5a (0.1 nM input concentration).
Applications
Application Human C5aR GPCR membrane preparation for Radioligand binding Assays.
Key Applications
  • Radioligand binding assay
Application Notes Radioligand binding assay (See images & graphs link)
Biological Information
Concentration 0.5 mg/mL
Entrez Gene Number
Gene Symbol
  • C5AR1
  • CD88
  • C5AR
  • C5A
  • C5R1
  • C5aR
  • C5a-R
GPCR Class A
Host Cells Chem-1
Protein Target C5aR
Target Sub-Family Anaphylotoxin
UniProt Number
UniProt Summary FUNCTION: SwissProt: P21730 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
SIZE: 350 amino acids; 39321 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
PTM: Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.
SIMILARITY: SwissProt: P21730 ## Belongs to the G-protein coupled receptor 1 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance SPECIFICATIONS: Bmax for [125I]C5a binding: 9.7 pmol/mg protein
Kd for [125I] C5a binding: ~ 0.59 nM
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Packaging Information
Material Size 200 assays
Transport Information
Supplemental Information
Specifications

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