ChemiScreen™ Membrane Prep Human Recombinant CCR10 - 炼石商城

您好！欢迎来到炼石商城请登录注册兼职小伙伴

我的购物车

0

Qiagen 凯杰 Biosharp Omega

全部商品分类

小伙伴店铺

客服热线 0771-3293894

首页重组蛋白Millipore 密理博>

图片仅供参考，请以实物为准

收藏

ChemiScreen™ Membrane Prep Human Recombinant CCR10

市场价：: 0.00

促销价：: ¥0.00

货号：HTS014M

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

亲，想了解价格请QQ咨询客服！

工作时间

周一至周五：9:00-18:00

咨询电话

0771-3293894

在线咨询

: 客服郭恒蔡玉坤曾宪飞技术咨询

浏览了该商品的用户最终购买

RNeasy Mini Kit (50) 50次

RNeasy Mini Kit (50) 50次

3720.00

Plasmid Mini Kit I(200) 200次

Plasmid Mini Kit I(200) 200...

576.00

10ul吸头，透明，袋装，可灭菌 1000支/袋，50袋/箱

10ul吸头，透明，袋装，可灭菌...

85.80

浏览了该商品的用户还浏览了

QIAfilter Plasmid Maxi Kit (10) -10

QIAfilter Plasmid Maxi Kit (...

3000.00

QIAamp DNA Blood Midi Kit (20) 20次

QIAamp DNA Blood Midi Kit (2...

2680.00

QIAamp UCP Pathogen Mini Kit (50)

QIAamp UCP Pathogen Mini Kit...

4112.00

商品介绍
商品咨询

Replacement Information

重要规格表

Species	Host Cells	Protein Target
Human	Chem-1	CCR10

Description
Catalogue Number	HTS014M
Brand Family	Chemicon®
Trade Name	ChemiScreen Chemicon
Description	ChemiSCREEN™ CCR10 Membrane Preparation
Overview	Full length human CCR10 with a proprietary mutation in the cytoplasmic domain to enhance cell surface expression.
Background Information	CCR10, previously known as GPR2, is a GPCR that is expressed in dermal endothelial cells, fibroblasts, melanocytes, and circulating T cells and PBMCs (Homey et al., 2000). Two chemokines, CTACK/CCL27 and MEC/CCL28, bind to CCR10 and stimulate migration and calcium flux (Pan et al., 2000; Wang et al., 2000). Expression of CTACK by keratinocytes, and CCR10 by lymphocytes from skin of patients with psoriasis and dermatitis, implicates this receptor ligand pair in skin inflammation. In addition, disruption of CCL27-CCR10 interaction reduces skin inflammation induced by allergen (Homey et al., 2002). CCR10 expressed on IgA antibody-secreting cells also mediates their migration to CCL28 expressed in the lactating mammary gland, and subsequent IgA accumulation in milk (Wilson and Butcher, 2004). Millipore's CCR10 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR10 interactions with its ligands. The membrane preparations exhibit an EC50 of 47 nM for CTACK/CCL27 in a GTPγS binding assay.

References

Product Information
Format	Membranes
Presentation	Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80^oC.

Applications
Application	Human CCR10 GPCR membrane preparation for GTPγS Binding Assays.
Key Applications	GTPγS binding
Application Notes	GTPγS Binding Assay.

Biological Information
Concentration	1.0 mg/mL
Entrez Gene Number	AF215981
Entrez Gene Summary	The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
Gene Symbol	CCR9 CC-CKR-9 D6 GPR-9-6 CDw199 CCBP2 GPR28 CCR10 CCR-9 CMKBR9
GPCR Class	A
Host Cells	Chem-1
Protein Target	CCR10
Target Sub-Family	Chemokine
UniProt Number	P51686
UniProt Summary	FUNCTION: SwissProt: P51686 # Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. SIZE: 369 amino acids; 42016 Da SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in the thymus and low in lymph nodes and spleen. SIMILARITY: SwissProt: P51686 ## Belongs to the G-protein coupled receptor 1 family. MISCELLANEOUS: EC50 of SCYA25/TECK for isoform 1 is lower than for isoform 2.
Incubation Conditions	GTPγS ASSAY CONDITIONS: Membranes are permeabilized by addition of saponin to an equal concentration by mass, then mixed with [³⁵S]-GTPγS (final concentration of 0.3 nM) in 20 mM HEPES, pH 7.4/100 mM NaCl/10 mM MgCl₂/0.5 μM GDP in a nonbinding 96-well plate. Unlabeled ligand was added to the final concentration indicated in Figure 1 (final volume 100 μL), and incubated for 30 min at 30°C. The binding reaction is transferred to an FB filter plate (Millipore MAHF B1H) previously prewetted with water. The plate is washed 3 times (1 mL per well per wash) with cold 10 mM sodium phosphate, pH 7.4, then dried and counted.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	EC₅₀ for CTACK/CCL27: ~5.6 nM in a GTPγS binding assay
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw.

Packaging Information
Material Size	200 assays

Transport Information

Supplemental Information

Specifications

登录 / 注册

购物指南免费注册批量采购购物流程购物保障

配送方式上门自提第三方配送炼石配送

支付方式货到付款账期付款

售后服务售后政策退款说明售后流程免责条款

服务热线

0771-3293894