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Hu Recombinant MCP-1 (MCAF)

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货号：GF012

品牌：Millipore 密理博

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Replacement Information

重要规格表

Key Applications Species Source Purity
CULT Human E.coli Greater than 99% by SDS-PAGE and HPLC analysis
Description
Catalogue Number GF012
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Monocyte Chemotactic Protein-1, Recombinant human
Overview Human macrophage chemoattractant protein-1 (MCP-1) also called human macrophage/monocyte chemotactic and activating factor (MCAF) is an 8.6 kDA protein containing 76 amino acids residues. It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.
Alternate Names
  • MCP-1
  • MCAF
References
Product Information
Presentation Lyophilized protein with no additives.
Applications
Application The human macrophage chemoattractant protein-1 (or MCP-1 protein) also called human macrophage/monocyte chemotactic & activating factor (MCAF) is an 8.6 kDA protein containing 76 amino acids residues.
Key Applications
  • Cell Culture
Biological Information
Purity Greater than 99% by SDS-PAGE and HPLC analysis
Source E.coli
Specific Activity The biological activity was determined by a chemotaxis assay using human monocytes. Significant chemotaxis response was found to be in the range of 10-100 ng/mL.
Entrez Gene Number
Entrez Gene Summary This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
Gene Symbol
  • CCL2
  • SMC-CF
  • MCAF
  • HSMCR30
  • GDCF-2
  • SCYA2
  • MCP1
  • HC11
  • MGC9434
  • MCP-1
UniProt Number
UniProt Summary FUNCTION: SwissProt: P13500 # Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
SIZE: 99 amino acids; 11025 Da
SUBUNIT: Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans.
SUBCELLULAR LOCATION: Secreted.
PTM: Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
SIMILARITY: SwissProt: P13500 ## Belongs to the intercrine beta (chemokine CC) family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute in water to a concentration of 0.1 mg/mL. This solution can be diluted into other buffered solutions. Reconstituted GF012 should be stored at -20°C in undiluted aliquots for up to six months.
Packaging Information
Material Size 20 µg
Transport Information
Supplemental Information
Specifications

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