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Qiagen 凯杰 Biosharp Omega

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Milliplex Human IL-4 Bead Set

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货号：SPR100

品牌：Millipore 密理博

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity Key Applications Host Format Antibody Type
H WB, ICC M Purified Monoclonal Antibody
Description
Catalogue Number MABC1037
Description Anti-Sp100 Antibody, clone 2H1.1
Alternate Names
  • Nuclear autoantigen Sp-100
  • Nuclear dot-associated Sp100 protein
  • Speckled 100 kDa
Background Information Nuclear autoantigen Sp-100 (UniProt P23497; also known as Nuclear dot-associated Sp100 protein, Speckled 100 kDa) is encoded by the SP100 gene (Gene ID 6672) in human. The SP100 family genes, including SP100, SP110, SP140 and SP140L, encode highly similar proteins mainly expressed in leukocytes. Interferons (IFNs) and other viral infection-related stimuli induce SP100 family gene expression and mutations of SP100 genes are linked to immune diseases and cancers. SP100 colocalizes with promyelocytic leukemia (PML) protein in nuclear bodies (NBs), also known as nuclear domain 10 (ND10), that function to regulate gene transcription via chromatin-based mechanisms and thereby mediate a variety of cellular functions including senescence, apoptosis, protein degradation, telemere stasbility, and defense against viral infections.
References
Product Information
Format Purified
Presentation Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-Sp100 Antibody, clone 2H1.1 is an antibody against Sp100 for use in Western Blotting, Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application Notes Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Sp100 nuclear foci in HeLa cells.
Biological Information
Immunogen GST-tagged recombinant human Sp100 internal fragment.
Epitope Internal (N-terminal half).
Clone 2H1.1
Concentration Please refer to lot specific datasheet.
Host Mouse
Specificity Clone 2H1.1 targeted epitope region is present in all seven human Sp100 spliced isoforms reported by UniProt (P23497).
Isotype IgG1κ
Species Reactivity
  • Human
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SP100
Purification Method Protein G purified.
UniProt Number
Molecular Weight ~100/78 kDa observed. 100.3 kDa (isoform Sp100-HMG/SP100HMG/SpAlt-HMG), 53.64 kDa (isoform Sp100-A/SP100A/SP100), 78.43 kDa (isoform Sp100-B/SP100B/SpAlt-212), 101.4 kDa (isoform Sp100-C/SP100C), 52.98 kDa (isoform SpAlt-C), 50.46 kDa (isoform 6), and 49.90 kDa (isoform 7) calculated (with Met1 removed). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in HepG2 nuclear extract.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Sp100 in 10 µg of HepG2 nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 μg
Transport Information
Supplemental Information
Specifications

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