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Milliplex Catalog ID.RV1MAG-26K-07.Rat Vascular Injury Mag 1

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货号：RV1MAG-26K-07

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Caveolin-1, CINC-1/GRO/KC, CTGF (Connective Tissue Growth Factor), IL-6, MCP-1, PAI-1 (total), TIMP-1, TNF-α, VEGF R Mplex Luminex xMAP
Description
Catalogue Number RV1MAG-26K
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Rat Vascular Injury Magnetic Bead Panel 1 - Toxicity Multiplex Assay
Overview The MILLIPLEX® MAP Rat Vascular Injury Magnetic Bead Panel contains all the components necessary to measure the following 9 biomarkers in any combination using Luminex® xMAP® technology: Caveolin-1, CINC-1/GRO/KC, CTGF (Connective Tissue Growth Factor), IL-6, MCP-1, PAI-1 (total), TIMP-1, TNFα, VEGF. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
Background Information Vascular injury is important to address during the preclinical safety study of drugs. A lack of well-defined mechanisms of drug-induced vascular injury (DIVI) in animals, and the absence of specific, qualified biomarkers have become significant barriers in the development of new therapeutic treatments. In the past, hemodynamics, such as heart rate and mean arterial blood pressure, were used to measure DIVI risks but these only targeted single biomarkers. Today, some of the known vascular injury mechanisms are related to vascular wall shear and/or circumferential “hoop” stress, drug/chemical toxicity, and immunological mediation. With these known biomechanics, primary targets (smooth muscle, endothelial and immune-mediated cells) yield a number of promising candidate biomarkers for DIVI study. We provide valuable research assays to investigate multiple biomarkers of vascular injury in rat serum and plasma samples using the Luminex xMAP® instrument platform.
References
Product Information
Detection method Luminex xMAP
Configuration Design your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: Caveolin-1 < 20%
  • Inter-assay: CINC-1/GRO/KC, PAI-1 (total), TIMP-1, TNFα < 15%
    All other analytes < 10%
  • Intra-assay: < 10%
Panel Type MAGNETIC Toxicity
Applications
Application The analytes available for this multiplex kit are: Caveolin-1, CINC-1/GRO/KC, CTGF (Connective Tissue Growth Factor), IL-6, MCP-1, PAI-1 (total), TIMP-1, TNFα, VEGF.
Key Applications
  • Multiplexing
Application Notes • This is an overnight assay.
• This assay requires 25 μL of 1:4 diluted serum or plasma sample per well in kit Serum Matrix (please dilute per protocol instructions).
Biological Information
Specificity There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity
  • Rat
Analytes Available
  • Caveolin-1
  • CINC-1/GRO/KC
  • CTGF (Connective Tissue Growth Factor)
  • IL-6
  • MCP-1
  • PAI-1 (total)
  • TIMP-1
  • TNF-α
  • VEGF
Assay Panel Everything you need in a single kit.
Cross Reactivity There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 97% - 108%
Physicochemical Information
Sensitivity
  • See kit protocol for individual analytes sensitivities.
Linearity of Dilution Contact technical support for this information
Standard Curve Range
  • Caveolin-1: 1.4 – 1,000 ng/mL
  • CINC-1/GRO/KC, IL-6, MCP-1, PAI-1 (total): 0.1 – 100 ng/mL
  • CTGF: 0.3 – 200 ng/mL
  • TIMP-1: 0.2 – 150 ng/mL
  • TNFα: 0.1 – 20 ng/mL
  • VEGF: 0.1 – 15 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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