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Qiagen 凯杰 Biosharp Omega

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Serum Matrix MILLIPLEX-COLD PACKS

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货号：LSM

品牌：Millipore 密理博

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Replacement Information

特卖

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
M WB, IP R Purified Monoclonal Antibody
Description
Catalogue Number MABE893
Description Anti-LSm1 Antibody, clone 5F3
Alternate Names
  • U6 snRNA-associated Sm-like protein LSm1
Background Information The protein named U6 snRNA-associated Sm-like protein LSm1 or Cancer associated Sm-like, or Small nuclear ribonuclear CaSm and encoded by the human gene LSM1/CASM is a critical binding protein that mediates replication dependent histone mRNA degradation during cell division. Lsm1 is part of the Lsm family of proteins. Lsm proteins are a ubiquitous family of proteins characterized by the Sm-domain. They exist as hexa- or heptameric RNA-binding complexes and carry out RNA-related functions. Lsm1 interacts directly with SLBP a histone stem-loop binding protein critical for histone mRNA processing and expression. Histone mRNAs do not contain introns or polyadenylation signals and are processed by direct endonucleolytic cleavage. Lsm1 binds directly to the 3’-terminal U-tract of U6 snRNA . Lsm1 is localized to the nucleus and is up regulated in many cancers including pancreatic cancer and many tumor cell lines.
References
Product Information
Format Purified
Presentation Purified rat monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application Anti-LSm1 Antibody, clone 5F3 is an antibody against LSm1 for use in western blotting & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application Notes Western Blotting Analysis: A representative lot from an independent laboratory detected LSm1 in MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LSm1 from MEF cell lysate (Hoefig, K. P., et al. (2012). Nat Struct Mol Biol. 20(1):73-81.).
Biological Information
Immunogen Recombinant protein corresponding to mouse LSm1.
Clone 5F3
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Rat
Isotype IgG1
Species Reactivity
  • Mouse
Antibody Type Monoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Lsm1
Purification Method Protein G Purified
UniProt Number
Molecular Weight ~15 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance Evaluated by Western Blotting in L-929 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected LSm1 in 10 µg of L-929 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size 100 µg
Transport Information
Supplemental Information
Specifications

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