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Assay Buffer for MILLIPLEX

市场价：: 0.00

促销价：: ¥0.00

货号：L-AB1

品牌：Millipore 密理博

配送至：

北京朝阳

支付：账期货到付款

数量：

EA (预计3-5工作日到货)

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Replacement Information

特卖

100% Performance Guaranteed

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
M	WB, IH(P), ACT	R	Purified	Monoclonal Antibody

Description
Catalogue Number	MABC950
Description	Anti-Aminopeptidase N/CD13 Antibody, clone SL13
Alternate Names	Aminopeptidase N/CD13 Aminopeptidase N AP-N mAPN Alanyl aminopeptidase Aminopeptidase M AP-M Membrane protein p161 Microsomal aminopeptidase CD13
Background Information	Aminopeptidase N (APN), also known as CD13 or Alanyl Aminopeptidase, is the protein product of the ANPEP gene. APN is a membrane-bound zinc metalloproteinase that catalyzes the cleavage of N-terminal amino acids and is involved in several biological processes including antigen processing and presentation, cell adhesion, tumor invasion and metastasis, neurotransmitter degradation. Widely expressed in many tissues and cells, including kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS, as well as in endometrial stromal cells, but not in the endometrial glandular cells. It is also found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. An elevation of the soluble form is found to be elevated in plasma and effusions of cancer patients. Elevated APN level in urine is also used in the diagnosis of kidney damages, such as that resulted from graft rejection after renal transplantation. APN is also known as a receptor for human coronavirus.

References

Product Information
Format	Purified
Presentation	Purified rat monoclonal IgG2bκ in buffer containing PBS without preservatives.

Applications
Application	Anti-Aminopeptidase N/CD13 Antibody, clone SL13 is an antibody against Aminopeptidase N/CD13 for use in Immunohistochemistry and Immunocytochemistry (Paraffin), Activity Assay.
Key Applications	Western Blotting Immunohistochemistry (Paraffin) Activity Assay
Application Notes	Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Aminopeptidase N/CD13 in mouse kidney cortex, mouse kidney medulla, and mouse colon tissue. Immunohistochemistry Analysis: A representative lot detected myocardial infarction-/MI-induced infiltration of aminopeptidase N/CD13-positive dendritic cells in murine cardiac tissue (Pereira, F.E., et al. (2013) Cardiovasc. Res. 100(1):74-83). Western Blotting Analysis: A representative lot detected aminopeptidase N/CD13 upregulation in murine cardiac tissue following myocardial infarction (MI) induction (Pereira, F.E., et al. (2013) Cardiovasc. Res. 100(1):74-83). Activity Assay Analysis: A representative lot observed Aminopeptidase N/CD13 blocking monocyte trafficking (Subramani, J., et al. (2014). J. Immunol. 191(7):3905-3912). Activity Assay Analysis: A representative lot observed Aminopeptidase N/CD13 blocks the adhesion to matrigel of WT mouse satellite cells (Rahman. M.M., et al. (2014). Stem Cells. 32(6):1564-1577). Western Blotting Analysis: A representative lot detected Aminopeptidase N/CD13 in primary muscle satellite cells (Rahman. M.M., et al. (2014). Stem Cells. 32(6):1564-1577).

Biological Information
Immunogen	Recombinant protein corresponding to mouse Aminopeptidase N/CD13.
Clone	SL13
Concentration	Please refer to lot specific datasheet.
Host	Rat
Isotype	IgG2bκ
Species Reactivity	Mouse
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_032512
Gene Symbol	Anpep Lap-1 Lap1
Purification Method	Protein G Purified
UniProt Number	P97449
Molecular Weight	~160 kDa observed. The calculated molecular weight is 110 kDa, however Aminopeptidase N/CD13 has been shown as a ~160 kDa band in western blots (Soderberg, et al. (1993). Journal of virology).

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in mouse kidney tissue lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Aminopeptidase N/CD13 in 10 µg of mouse kidney tissue lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

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