Milliplex Catalog ID.HTH17MAG-14K-24.Human Th17 Panel MAGNETIC

Analytes Available	Species Reactivity	Key Applications	Detection Methods
GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ	H	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ

Mplex

Luminex xMAP

Description
Catalogue Number	HTH17MAG-14K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay
Overview	EMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel MILLIPLEX® MAP offers you: o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs. o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay. EMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information	CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation. Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth. The MILLIPLEX® MAP Human TH17 Magnetic Bead Panell enables you to focus on the relevance and therapeutic potential of the modulation of CD4 T-helper cell cytokines in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

Description

Catalogue Number

HTH17MAG-14K

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Human TH17 Magnetic Bead Panel - Immunology Multiplex Assay

Overview

EMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:

• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel

MILLIPLEX® MAP offers you:
o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

EMD Millipore’s MILLIPLEX® MAP Human TH17 Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

Background Information

CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn's disease and autoimmune uveitis. In addition Th17 cells play an important role in tumor development, progression and metastasis, potentially in both promoting and inhibiting tumor growth.

The MILLIPLEX® MAP Human TH17 Magnetic Bead Panell enables you to focus on the relevance and therapeutic potential of the modulation of CD4 T-helper cell cytokines in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Inter-assay: < 15 Intra-assay:< 10
Panel Type	MAGNETIC Cytokines/Chemokines

Product Information

Detection method

Luminex xMAP

Configuration

Design your multiplex kit by choosing available analytes within this panel.

Precision, %

Inter-assay: < 15
Intra-assay:< 10

Panel Type

MAGNETIC Cytokines/Chemokines

Applications
Application	Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well. • This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant

Applications

Application

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

Key Applications

Multiplexing

Application Notes

• This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Th17 panel analytes in serum, plasma or tissue/cell lysate and culture supernatant

Biological Information
Species Reactivity	Human
Analytes Available	GM-CSF IFN-γ IL-1β IL-2 IL-4 IL-5 IL-6 IL-9 IL-10 IL-12 (p70) IL-13 IL-15 IL-17A IL-17F IL-17E/IL-25 IL-21 IL-22 IL-23 IL-27 IL-28A IL-31 IL-33 MIP-3α/CCL20 TNF-α TNFβ
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	79 - 107%

Biological Information

Species Reactivity

Human

Analytes Available

GM-CSF
IFN-γ
IL-1β
IL-2
IL-4
IL-5
IL-6
IL-9
IL-10
IL-12 (p70)
IL-13
IL-15
IL-17A
IL-17F
IL-17E/IL-25
IL-21
IL-22
IL-23
IL-27
IL-28A
IL-31
IL-33
MIP-3α/CCL20
TNF-α
TNFβ

Cross Reactivity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Accuracy

79 - 107%

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range	IL-17 & IL-4, 24 - 100,000 pg/mL GM-CSF & IL-27, 61 - 250,000 pg/mL IFNγ, 10 - 40,000 pg/mL IL-10, 1 - 5000 pg/mL MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33, 5 - 20,000 pg/mL IL-13, 7 - 30,000 pg/mL IL-17A & IL-2, 12 - 50,000 pg/mL IL-22 & TNFβ, 37 - 150,000 pg/mL IL-9, 9 - 35,000 pg/mL IL-23, 336 - 1,500,000 pg/mL IL-5, 6 - 25,000 pg/mL IL-6 & TNFα, 3 - 10,000 pg/mL IL-17E/IL-25, 488 - 2,000,000 IL-28a & IL-31, 49 - 200,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitivities of individual biomarkers.

Standard Curve Range

IL-17 & IL-4, 24 - 100,000 pg/mL
GM-CSF & IL-27, 61 - 250,000 pg/mL
IFNγ, 10 - 40,000 pg/mL
IL-10, 1 - 5000 pg/mL
MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33, 5 - 20,000 pg/mL
IL-13, 7 - 30,000 pg/mL
IL-17A & IL-2, 12 - 50,000 pg/mL
IL-22 & TNFβ, 37 - 150,000 pg/mL
IL-9, 9 - 35,000 pg/mL
IL-23, 336 - 1,500,000 pg/mL
IL-5, 6 - 25,000 pg/mL
IL-6 & TNFα, 3 - 10,000 pg/mL
IL-17E/IL-25, 488 - 2,000,000
IL-28a & IL-31, 49 - 200,000 pg/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Packaging Information

Material Size

96-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Milliplex Catalog ID.HTH17MAG-14K-24.Human Th17 Panel MAGNETIC

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