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MILLIPLEX Human High Sensitivity T Cell Magnetic Bead 13 Plex PREMIXED Kit

市场价：
31.00
促销价：
¥0.00

货号：HSTCMAG28SPMX13

品牌：Millipore 密理博

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Replacement Information
Replacement Information MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 13-plex, HSTCMAG28SPMX13 replaces MILLIPLEX MAP High Sensitivity Human Cytokine Magnetic Bead Panel, HSCYMAG60SPMX13

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, TNF-α H Mplex Luminex xMAP
Description
Catalogue Number HSTCMAG28SPMX13
Replaces HSCYMAG60SPMX13
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Human High Sensitivity T Cell Panel Premixed 13-plex - Immunology Multiplex Assay
Background Information Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
References
Product Information
Detection method Luminex xMAP
Configuration Premixed
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel Type MAGNETIC Cytokines/Chemokines
Applications
Application Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes • This is an overnight incubation.
• Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay. These features are summarized on the next page.
• This kit may be used for the analysis of all or any combination of the Human High Sensitivity T Cell panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • GM-CSF
  • IFNγ
  • IL-1β
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-12 (p70)
  • IL-13
  • TNF-α
Cross Reactivity There is no detectable cross-reactivity within the panel.
Accuracy
  • 98% – 107%
Physicochemical Information
Sensitivity
  • 0.11 - 8.17 pg/mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of Dilution Please contact technical service for information
Standard Curve Range
  • GM-CSF: 1.22 – 5,000 pg/mL
  • IFNγ: 0.61 – 2,500 pg/mL
  • IL-1β: 0.49 – 2,000 pg/mL
  • IL-2: 0.49 – 2,000 pg/mL
  • IL-4: 1.83 – 7,500 pg/mL
  • IL-5: 0.49 – 2,000 pg/mL
  • IL-6: 0.18 – 750 pg/mL
  • IL-7: 0.37 – 1,500 pg/mL
  • IL-8: 0.31 – 1,250 pg/mL
  • IL-10: 1.46 – 6,000 pg/mL
  • IL-12 (p70): 0.49 – 2,000 pg/mL
  • IL-13: 0.24 – 1,000 pg/mL
  • TNFα: 0.45 – 1,750 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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