Milliplex Catalog ID.HMYOMAG-56K-09.Human Myokine Magnetic

Analytes Available	Species Reactivity	Key Applications	Detection Methods
Apelin, BDNF, Erythropoietin (EPO), FABP3, FGF-21, Fractalkine/CX3CL1, Follistatin-like Protein 1 (FSTL1), IL-6, IL-15, Irisin, LIF, Myostatin/GDF8, Oncostatin M (OSM), Osteocrin/Musclin, Osteonectin (SPARC)	H	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

Apelin, BDNF, Erythropoietin (EPO), FABP3, FGF-21, Fractalkine/CX3CL1, Follistatin-like Protein 1 (FSTL1), IL-6, IL-15, Irisin, LIF, Myostatin/GDF8, Oncostatin M (OSM), Osteocrin/Musclin, Osteonectin (SPARC)

Mplex

Luminex xMAP

Description
Catalogue Number	HMYOMAG-56K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human Myokine Magnetic Bead Panel
Overview	EMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel 15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum and plasma samples and in cell/tissue supernatants: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel EMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information	Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only can act in autocrine and/or paracrine manner in regulation of skeletal muscle growth, they can also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations to the exercise-induced metabolic changes in liver and adipose tissue as well as the profound changes in immune and neuron systems. The MILLIPLEX® MAP Human Myokine Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1) in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

Description

Catalogue Number

HMYOMAG-56K

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Human Myokine Magnetic Bead Panel

Overview

EMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel 15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum and plasma samples and in cell/tissue supernatants: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1).

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:

• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel

EMD Millipore’s MILLIPLEX® MAP Human Myokine Magnetic Bead Panel is part of the most versatile system available for cytokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

Background Information

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only can act in autocrine and/or paracrine manner in regulation of skeletal muscle growth, they can also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations to the exercise-induced metabolic changes in liver and adipose tissue as well as the profound changes in immune and neuron systems.

The MILLIPLEX® MAP Human Myokine Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1) in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Apelin: Intra-assay <10% CV Inter-assay <15% BDNF: Intra-assay <10% CV Inter-assay <15% Erythropoietin (EPO): Intra-assay <10% CV Inter-assay <15% FABP3: Intra-assay <10% CV Inter-assay <15% FGF-21: Intra-assay <10% CV Inter-assay <15% Fractalkine/CX3CL1: Intra-assay <10% CV Inter-assay <15% Follistatin-like Protein 1 (FSTL1): Intra-assay <10% CV Inter-assay <15% IL-6: Intra-assay <10% CV Inter-assay <15% IL-15: Intra-assay <10% CV Inter-assay <15% Irisin: Intra-assay <10% CV Inter-assay <15% LIF: Intra-assay <10% CV Inter-assay <15% Myostatin/GDF8: Intra-assay <10% CV Inter-assay <15% Oncostatin M (OSM): Intra-assay <10% CV Inter-assay <15% Osteocrin/Musclin: Intra-assay <10% CV Inter-assay <15% Osteonectin (SPARC): Intra-assay <10% CV Inter-assay <20%
Panel Type	MAGNETIC Cytokines/Chemokines

Product Information

Detection method

Luminex xMAP

Configuration

Design your multiplex kit by choosing available analytes within this panel.

Precision, %

Apelin: Intra-assay <10% CV Inter-assay <15%
BDNF: Intra-assay <10% CV Inter-assay <15%
Erythropoietin (EPO): Intra-assay <10% CV Inter-assay <15%
FABP3: Intra-assay <10% CV Inter-assay <15%
FGF-21: Intra-assay <10% CV Inter-assay <15%
Fractalkine/CX3CL1: Intra-assay <10% CV Inter-assay <15%
Follistatin-like Protein 1 (FSTL1): Intra-assay <10% CV Inter-assay <15%
IL-6: Intra-assay <10% CV Inter-assay <15%
IL-15: Intra-assay <10% CV Inter-assay <15%
Irisin: Intra-assay <10% CV Inter-assay <15%
LIF: Intra-assay <10% CV Inter-assay <15%
Myostatin/GDF8: Intra-assay <10% CV Inter-assay <15%
Oncostatin M (OSM): Intra-assay <10% CV Inter-assay <15%
Osteocrin/Musclin: Intra-assay <10% CV Inter-assay <15%
Osteonectin (SPARC): Intra-assay <10% CV Inter-assay <20%

Panel Type

MAGNETIC Cytokines/Chemokines

Applications
Application	The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.
Key Applications	Multiplexing
Application Notes	Although a two hour sample incubation may be used, for optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.

Applications

Application

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

Key Applications

Multiplexing

Application Notes

Although a two hour sample incubation may be used, for optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Human
Analytes Available	Apelin BDNF Erythropoietin (EPO) FABP3 FGF-21 Fractalkine/CX3CL1 Follistatin-like Protein 1 (FSTL1) IL-6 IL-15 Irisin LIF Myostatin/GDF8 Oncostatin M (OSM) Osteocrin/Musclin Osteonectin (SPARC)
Assay Panel	Everything you need in a single kit.
Cross Reactivity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy	Apelin 100% BDNF 97% EPO 100% FABP3 79% FGF21 98% Fractalkine 97% FSTL-1 96% IL-6 82% IL-15 92% Irisin 96% LIF 97% Myostatin/GDF8 94% Oncostatin M (OSM) 98% Osteocrin/Musclin 97% Osteonectin (SPARC) 96%

Biological Information

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Species Reactivity

Human

Analytes Available

Apelin
BDNF
Erythropoietin (EPO)
FABP3
FGF-21
Fractalkine/CX3CL1
Follistatin-like Protein 1 (FSTL1)
IL-6
IL-15
Irisin
LIF
Myostatin/GDF8
Oncostatin M (OSM)
Osteocrin/Musclin
Osteonectin (SPARC)

Assay Panel

Everything you need in a single kit.

Cross Reactivity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.

Accuracy

Apelin 100%
BDNF 97%
EPO 100%
FABP3 79%
FGF21 98%
Fractalkine 97%
FSTL-1 96%
IL-6 82%
IL-15 92%
Irisin 96%
LIF 97%
Myostatin/GDF8 94%
Oncostatin M (OSM) 98%
Osteocrin/Musclin 97%
Osteonectin (SPARC) 96%

Physicochemical Information
Sensitivity	MinDC+2SD Apelin: 64 pg/mL BDNF: 9 pg/mL Erythropoietin (EPO): 815 pg/mL FABP3: 9.8 pg/mL FGF-21: 14 pg/mL Fractalkine/CX3CL1: 40 pg/mL Follistatin-like Protein 1 (FSTL1): 737 pg/mL IL-6: 0.9 pg/mL IL-15: 5 pg/mL Irisin: 281 pg/mL LIF: 4 pg/mL Myostatin/GDF8: 259 pg/mL Oncostatin M (OSM): 6 pg/mL Osteocrin/Musclin: 59 pg/mL Osteonectin (SPARC): 7.5 ng/mL
Linearity of Dilution	Contact technical support for this information
Standard Curve Range	Apelin: 122 - 500,000 pg/mL BDNF: 2 - 10,000 pg/mL Erythropoietin (EPO): 244 - 1,000,000 pg/mL FABP3: 24 - 100,000 pg/mL FGF-21: 5 - 20,000 pg/mL Fractalkine/CX3CL1: 49 - 200,000 pg/mL Follistatin-like Protein 1 (FSTL1): 488 - 2,000,000 pg/mL IL-6: 0.6 - 2,400 pg/mL IL-15: 2 - 10,000 pg/mL Irisin: 244 - 1,000,000 pg/mL LIF: 10 - 40,000 pg/mL Myostatin/GDF8: 488 - 2,000,000 pg/mL Oncostatin M (OSM): 2 -6,000 pg/mL Osteocrin/Musclin: 49 - 200,000 pg/mL Osteonectin (SPARC): 7 - 30,000 ng/mL

Physicochemical Information

Sensitivity

MinDC+2SD
Apelin: 64 pg/mL
BDNF: 9 pg/mL
Erythropoietin (EPO): 815 pg/mL
FABP3: 9.8 pg/mL
FGF-21: 14 pg/mL
Fractalkine/CX3CL1: 40 pg/mL
Follistatin-like Protein 1 (FSTL1): 737 pg/mL
IL-6: 0.9 pg/mL
IL-15: 5 pg/mL
Irisin: 281 pg/mL
LIF: 4 pg/mL
Myostatin/GDF8: 259 pg/mL
Oncostatin M (OSM): 6 pg/mL
Osteocrin/Musclin: 59 pg/mL
Osteonectin (SPARC): 7.5 ng/mL

Linearity of Dilution

Contact technical support for this information

Standard Curve Range

Apelin: 122 - 500,000 pg/mL
BDNF: 2 - 10,000 pg/mL
Erythropoietin (EPO): 244 - 1,000,000 pg/mL
FABP3: 24 - 100,000 pg/mL
FGF-21: 5 - 20,000 pg/mL
Fractalkine/CX3CL1: 49 - 200,000 pg/mL
Follistatin-like Protein 1 (FSTL1): 488 - 2,000,000 pg/mL
IL-6: 0.6 - 2,400 pg/mL
IL-15: 2 - 10,000 pg/mL
Irisin: 244 - 1,000,000 pg/mL
LIF: 10 - 40,000 pg/mL
Myostatin/GDF8: 488 - 2,000,000 pg/mL
Oncostatin M (OSM): 2 -6,000 pg/mL
Osteocrin/Musclin: 49 - 200,000 pg/mL
Osteonectin (SPARC): 7 - 30,000 ng/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Packaging Information

Material Size

96-well plate

Material Package

96 well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Milliplex Catalog ID.HMYOMAG-56K-09.Human Myokine Magnetic

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