Milliplex Catalog ID.HLPPMAG-57K-02.Human Liver Protein Magnetic

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Milliplex Catalog ID.HLPPMAG-57K-02.Human Liver Protein Magnetic

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货号：HLPPMAG-57K-02

品牌：Millipore 密理博

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北京朝阳

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Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1, FGF-19, FGF-21, FGF-23, HGF	H	Mplex	Luminex xMAP

Description
Catalogue Number	HLPPMAG-57K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human Liver Protein Magnetic Bead Panel - Metabolism Multiplex Assay
Overview	Millipore’s MILLIPLEX MAP Human Liver Protein Panel is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue/cell culture samples: AFP, ANGPTL3, ANGPTL4, ANGPTL6/AGF, FABP1/L-FABP, FGF-19, FGF-21, FGF-23 and HGF. NOTE: When assaying ANGPTL6/AGF it is recommended that serum samples be used.
Background Information	Liver-secreted proteins play important roles in metabolic regulation. For example, liver-secreted proteins have been shown to regulate circulating lipoprotein levels, energy expenditure, glucose metabolism, and fatty acid uptake. In addition, some liver-secreted proteins may also serve as biomarkers for liver diseases and gastric cancer. Accurate measurement of liver proteins is critical to obtain understanding of their biological functions.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	AFP: Intra-assay <10% CV Inter-assay <20% ANGPTL3: Intra-assay <10% CV Inter-assay <20% ANGPTL4: Intra-assay <10% CV Inter-assay <20% ANGPTL6/AGF: Intra-assay <10% CV Inter-assay <20% FABP1: Intra-assay <10% CV Inter-assay <20% FGF-19: Intra-assay <10% CV Inter-assay <20% FGF-21: Intra-assay <10% CV Inter-assay <20% FGF-23: Intra-assay <10% CV Inter-assay <20% HGF: Intra-assay <10% CV Inter-assay <20%
Panel Type	MAGNETIC Metabolism

Applications
Application	The Human Liver Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 9 liver protein biomarkers in human serum, plasma and tissue/cell culture samples.
Key Applications	Multiplexing
Application Notes	A 2 hour sample incubaton may be used, however, for optimal sensitivity, use an overnight (16-18 hour) incubation. Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.

Biological Information
Specificity	The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Species Reactivity	Human
Analytes Available	AFP ANGPTL3 ANGPTL4 ANGPTL6/AGF FABP1 FGF-19 FGF-21 FGF-23 HGF
Assay Panel	Everything you need in a single kit.
Cross Reactivity	The ANGPTL6 standard had cross reactivity with the ANGPTL4 assay of less than 2%. For all other analytes, there was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy	AFP 94.2% ANGPTL3 93.7% ANGPTL4 96.8% ANGPTL6 99.1% FABP1 96.6% FGF-19 98.0% FGF-21 98.8% FGF-23 96.6% HGF 100.2%

Physicochemical Information
Sensitivity	MinDC+2SD AFP: 0.144 ng/mL ANGPTL3: 0.725 ng/mL ANGPTL4: 2.150 ng/mL ANGPTL6: 0.847 ng/mL FABP1: 0.218 ng/mL FGF-19: 0.054 ng/mL FGF-21: 0.004 ng/mL FGF-23: 0.014 ng/mL HGF: 0.018 ng/mL
Linearity of Dilution	Contact technical support for this information
Standard Curve Range	AFP: 0.14 - 100 ng/mL ANGPTL3: 0.69 - 500 ng/mL ANGPTL4: 5.49 - 4000 ng/mL ANGPTL6/AGF: 1.37 - 1000 ng/mL FABP1: 0.27 - 200 ng/mL FGF-19: 0.14 - 100 ng/mL FGF-21: 0.01 - 10 ng/mL FGF-23: 0.04 - 30 ng/mL HGF: 0.07 - 50 ng/mL

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Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

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Milliplex Catalog ID.HLPPMAG-57K-02.Human Liver Protein Magnetic

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