Milliplex Catalog ID.HIGFBMAG-53K-03.Human (IGFBP) Magnetic

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Milliplex Catalog ID.HIGFBMAG-53K-03.Human (IGFBP) Magnetic

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货号：HIGFBMAG-53K-03

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Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGFBP-7	H	Mplex	Luminex xMAP

Description
Catalogue Number	HIGFBMAG-53K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human IGF Binding Protein Magnetic Bead Panel - Metabolism Multiplex Assay
Overview	The MILLIPLEX MAP Human IGF Binding Protein (IGFBP) Magnetic Bead Panel, is used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, and cell culture conditioned-media samples: IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, and IGFBP-7
Background Information	Insulin-like growth factor (IGF) binding proteins (IGFBP) are expressed by multiple tissues and cells, including hepatic cells. By binding both IGF-1 and IGF-2, IGFBPs play a critical role in the regulation of IGF action, altering IGF interaction with cell surface receptors and increasing circulating IGF half-life and bioavailability. While their functions at the cellular level are not fully understood, studies have demonstrated the capacity to both stimulate and inhibit IGF-1 activity, depending on the system involved and the IGFBP studied. The ability of IGFBPs to regulate cell sensitivity by inhibiting IGF signaling may function in the control of tumor cell growth.

References

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	IGFBP-1: Intra-assay <10% C Inter-assay <15% IGFBP-2: Intra-assay <10% CV Inter-assay <15% IGFBP-3: Intra-assay <10% CV Inter-assay <15% IGFBP-4: Intra-assay <10% CV Inter-assay <15% IGFBP-5: Intra-assay <10% CV Inter-assay <15% IGFBP-6: Intra-assay <10% CV Inter-assay <15% IGFBP-7: Intra-assay <10% CV Inter-assay <15%
Panel Type	MAGNETIC Endocrine

Applications
Application	The Human IGF Binding Protein Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 7 IGFBP protein biomarkers in human serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• To prevent possible IGFBP proteolysis, it is recommended that a protease inhibitor cocktail solubion be added to each blood sample after blood collection. • A 2-hour sample incubation may be used, however, for optimal sensitivity, use an overnight (16-18 hour) incubation. • Serum and plasma samples require (recommend) a 1:25 dilution with Assay Buffer. • Tissue culture supernatants may be run neat or appropriately diluted in control medium. Each well requires 25 μL of sample, standard or control.

Biological Information
Specificity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Species Reactivity	Human
Analytes Available	IGFBP-1 IGFBP-2 IGFBP-3 IGFBP-4 IGFBP-5 IGFBP-6 IGFBP-7
Assay Panel	Everything you need in a single kit.
Cross Reactivity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy	IGFBP-1 102% IGFBP-2 90% IGFBP-3 95% IGFBP-4 97% IGFBP-5 79% IGFBP-6 105% IGFBP-7 98%

Physicochemical Information
Sensitivity	MinDC+2SD IGFBP-1: 0.01 ng/mL IGFBP-2: 1.09 ng/mL IGFBP-3: 0.15 ng/mL IGFBP-4: 0.96 ng/mL IGFBP-5: 9.87 ng/mL IGFBP-6: 0.12 ng/mL IGFBP-7: 0.07 ng/mL
Linearity of Dilution	Contact technical support for this information
Standard Curve Range	IGFBP-1: 0.03 - 25 ng/mL IGFBP-2: 0.34 - 250 ng/mL IGFBP-3: 0.34 - 250 ng/mL IGFBP-4: 0.69 - 500 ng/mL IGFBP-5: 1.37 - 1000 ng/mL IGFBP-6: 0.14 - 100 ng/mL IGFBP-7: 0.07 - 50 ng/mL

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Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate
Material Package	96 well plate

Transport Information

Supplemental Information

Specifications

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Milliplex Catalog ID.HIGFBMAG-53K-03.Human (IGFBP) Magnetic

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重要规格表

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