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MILLIPLEX Human IgE Magnetic Bead Single Plex Kit

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货号：HGAMMAG-303E

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
IgE H Mplex Luminex xMAP
Description
Catalogue Number HGAMMAG-303E
Trade Name
  • MILLIPLEX
Description MILLIPLEX® MAP Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay
Background Information Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions, and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found
primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins and leukotrienes, resulting in an “allergic reaction”.

Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX MAP Human IgE Magnetic Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.
References
Product Information
Components
  • MILLIPLEX® MAP Anti-Human k and λ Light Chain, PE
  • MILLIPLEX® MAP Human IgE Standard
  • MILLIPLEX® MAP Human Serum Positive Control
  • MILLIPLEX® MAP Assay Buffer
  • MILLIPLEX® MAP Washer Buffer, 10X
  • Set of one 96-Well Filter Plate with 4 Sealers
Detection method Luminex xMAP
Configuration Single Plex
Precision, %
  • Intra-Assay < 10
  • Inter-Assay < 13
Panel Type MAGNETIC Immune Response
Applications
Application Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes • This kit may be used for the analysis of IgE in human serum, plasma or tissue/cell lysate and culture supernatant samples.
• There are 2 one-hour room-temperature incubations.
• This assay requires 50 µl of 1:50 diluted plasma or serum, or 50 μL cell culture supernatant per well.
Biological Information
Specificity Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • IgE
Accuracy
  • IgE 102%
Physicochemical Information
Sensitivity
  • 0.4 ng/mL
Standard Curve Range
  • IgE 4.1 – 1,000 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size 96-well plate
Material Package 96 Well Plate
Transport Information
Supplemental Information
Specifications

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