Milliplex Catalog ID.HCCB4MAG-58K-04.MAG Circulating Cancer Panel 4

Analytes Available	Species Reactivity	Key Applications	Detection Methods
ALDH1A1, CD44, Hepsin, L1CAM, Mesothelin, Transglutaminase 2 (TGM2), Carbonic Anhydrase IX (CA9), EpCam, Kallikrein-6, Midkine	H	Mplex	Luminex xMAP

Analytes Available

Species Reactivity

Key Applications

Detection Methods

ALDH1A1, CD44, Hepsin, L1CAM, Mesothelin, Transglutaminase 2 (TGM2), Carbonic Anhydrase IX (CA9), EpCam, Kallikrein-6, Midkine

Mplex

Luminex xMAP

Description
Catalogue Number	HCCB4MAG-58K
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay
Overview	EMD Millipore’s MILLIPLEX® MAPHuman Circulating Cancer Biomarker Magnetic Bead Panel4 to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples:ALDH1A1, CD44, hepsin, L1CAM, mesothelin, transglutaminase 2 (TGM2), carbonic anhydrase IX (CA9), epCam, kallikrein-6, and midkine. MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. Each MILLIPLEX® MAP panel and kit includes: • Quality controls (QCs) provided to qualify assay performance • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency • Optimized serum matrix to mimic native analyte environment • Detection antibody cocktails designed to yield consistent analyte profiles within panel MILLIPLEX® MAP offers you: o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs. o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay. EMD Millipore’s MILLIPLEX® MAP Human Diabetes Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background Information	Cancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include extracellular cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

Description

Catalogue Number

HCCB4MAG-58K

Trade Name

MILLIPLEX

Description

MILLIPLEX MAP Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

Overview

EMD Millipore’s MILLIPLEX® MAPHuman Circulating Cancer Biomarker Magnetic Bead Panel4 to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples:ALDH1A1, CD44, hepsin, L1CAM, mesothelin, transglutaminase 2 (TGM2), carbonic anhydrase IX (CA9), epCam, kallikrein-6, and midkine.

MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® MAP panel and kit includes:

• Quality controls (QCs) provided to qualify assay performance

• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

• Optimized serum matrix to mimic native analyte environment

• Detection antibody cocktails designed to yield consistent analyte profiles within panel

MILLIPLEX® MAP offers you:
o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

EMD Millipore’s MILLIPLEX® MAP Human Diabetes Magnetic Bead Panel is part of the most versatile system available for complement factor research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.

Background Information

Cancer research has always focused on intracellular biomarkers, such as HER2 and B-Raf, and in recent years, more and more attention has shifted to include extracellular cancer biomarkers. While the use of intracellular markers in research helps clarify the process of oncogenesis, circulating cancer biomarkers give insight into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Study of isolated biomarkers, whether intracellular or circulating, is often inadequate to fully characterize the complex relationship between tumor and non-tumor. Consequently, multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

Product Information
Detection method	Luminex xMAP
Configuration	Design your multiplex kit by choosing available analytes within this panel.
Precision, %	Intra-assay: < 20% Inter-assay: < 10
Panel Type	MAGNETIC Circulating Cancer

Product Information

Detection method

Luminex xMAP

Configuration

Design your multiplex kit by choosing available analytes within this panel.

Precision, %

Intra-assay: < 20%
Inter-assay: < 10

Panel Type

MAGNETIC Circulating Cancer

Applications
Application	MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP® technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.
Key Applications	Multiplexing
Application Notes	This is an overnight incubation assay. A maximum of 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay. This kit may be used for the analysis of all or any combination of the Human Circulating Cancer Biomarker panel 4 analytes in serum, plasma or tissue/cell/tumor culture supernatant. Please note that min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL.

Applications

Application

MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP® technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

Key Applications

Multiplexing

Application Notes

This is an overnight incubation assay.

A maximum of 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay.

This kit may be used for the analysis of all or any combination of the Human Circulating Cancer Biomarker panel 4 analytes in serum, plasma or tissue/cell/tumor culture supernatant.

Please note that min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL.

Biological Information
Specificity	There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Species Reactivity	Human
Analytes Available	ALDH1A1 CD44 Hepsin L1CAM Mesothelin Transglutaminase 2 (TGM2) Carbonic Anhydrase IX (CA9) EpCam Kallikrein-6 Midkine
Assay Panel	Everything you need in a single kit.
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	80%-110%

Biological Information

Specificity

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Species Reactivity

Human

Analytes Available

ALDH1A1
CD44
Hepsin
L1CAM
Mesothelin
Transglutaminase 2 (TGM2)
Carbonic Anhydrase IX (CA9)
EpCam
Kallikrein-6
Midkine

Assay Panel

Everything you need in a single kit.

Cross Reactivity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Accuracy

80%-110%

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities of individual biomarkers.
Linearity of Dilution	Contact technical support for this information
Standard Curve Range	ALDH1A1: 2.7 - 2,000 ng/mL CD44: 1.4 - 1,000 ng/mL Hepsin: 0.3 - 250 ng/mL L1CAM: 0.1 - 100 ng/mL Mesothelin: 0.1 - 100 ng/mL Transglutaminase 2 (TGM2): 1.4 - 1,000 ng/mL Carbonic Anhydrase IX (CA9): 20.6 - 15,000 pg/mL EpCAM: 13.7 - 10,000 pg/mL Kallekrein-6: 27.4-20,000 pg/mL Midkine: 13.7 - 10,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitivities of individual biomarkers.

Linearity of Dilution

Contact technical support for this information

Standard Curve Range

ALDH1A1: 2.7 - 2,000 ng/mL
CD44: 1.4 - 1,000 ng/mL
Hepsin: 0.3 - 250 ng/mL
L1CAM: 0.1 - 100 ng/mL
Mesothelin: 0.1 - 100 ng/mL
Transglutaminase 2 (TGM2): 1.4 - 1,000 ng/mL
Carbonic Anhydrase IX (CA9): 20.6 - 15,000 pg/mL
EpCAM: 13.7 - 10,000 pg/mL
Kallekrein-6: 27.4-20,000 pg/mL
Midkine: 13.7 - 10,000 pg/mL

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Storage and Shipping Information

Storage Conditions

Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Packaging Information

Material Size

96-well plate

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

办公用品

Milliplex Catalog ID.HCCB4MAG-58K-04.MAG Circulating Cancer Panel 4

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