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10-Plex MAPK/SAPK Signaling Magnetic Bead Kit

市场价：
25.00
促销价：
¥0.00

货号：48-660MAG

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
ATF2 (Thr71), Erk (Thr185/Tyr187), HSP27 (Ser78), JNK (Thr183/Tyr185), c-Jun (Ser73), MEK1 (Ser222), MSK1 (Ser212), p38 (Thr180/Tyr182), p53 (Ser15), STAT1 (Tyr701) H Mplex Luminex xMAP
Description
Catalogue Number 48-660MAG
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay
Overview Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.
References
Product Information
Detection method Luminex xMAP
Configuration Premixed
Precision, %
  • Intra-Assay: 3% - 10%
  • Inter-Assay: 4% - 13%
Panel Type Cell Signaling
Applications
Application Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.
Key Applications
  • Multiplexing
Application Notes • An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 µL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).
Biological Information
Specificity Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • ATF2 (Thr71)
  • Erk (Thr185/Tyr187)
  • HSP27 (Ser78)
  • JNK (Thr183/Tyr185)
  • c-Jun (Ser73)
  • MEK1 (Ser222)
  • MSK1 (Ser212)
  • p38 (Thr180/Tyr182)
  • p53 (Ser15)
  • STAT1 (Tyr701)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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