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Phospho STAT1 (Tyr701) Magnetic Bead MAPmate (ship set)

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货号：46-655MAG

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
STAT1 (Tyr701) H, M Mplex Luminex xMAP
Description
Catalogue Number 46-655MAG
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Phospho STAT1 (Tyr701) Magnetic Bead MAPmate - Cell Signaling Single Plex Assay
Materials Required but Not Delivered • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
References
Product Information
Detection method Luminex xMAP
Configuration Single Plex
Panel Type Cell Signaling
Applications
Application MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is STAT1 (Tyr701).
Key Applications
  • Multiplexing
Application Notes • An overnight (4°C) incubation is recommended for best results
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • STAT1 (Tyr701)
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
Modifications
  • Phosphorylation
UniProt Number
UniProt Summary Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C
Packaging Information
Material Size 96-well plate
Material Package 96-well Plate
Transport Information
Supplemental Information
Specifications

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