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Phospho-p38/SAPK Magnetic Bead MAPmates

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号:46-610AMAG

牌:Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
p38α/SAPK2 (Thr180/Tyr182) H, M, R Mplex Luminex xMAP
Description
Catalogue Number 46-610MAG
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay
Overview The MILLIPLEX® MAP Phospho p38α/SAPK2 (Thr180/Tyr182) Magnetic Bead MAPmate™ pair is used in conjunction with the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG) to detect the presence of Phospho p38α/SAPK2 (Thr180/Tyr182) in cell lysates using the Luminex 100™ IS, 200™, HTS, FLEXMAP 3D®, MAGPIX® system. Each MAPmate™ pair is ordered individually and may be combined for simultaneous multiplex analysis of cellular events. The MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit is ordered separately and consists of a common set of reagents needed for performing MAPmate™ assays. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEX® MAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit may be utilized as an unstimulated control for this target, while the HeLa Cell Lysate: HS/Ars included with this MAPmate™ may be utilized as a stimulated control.

Important note: For a detailed protocol on Cell Signaling Detection Procedures please see the instruction booklet for the MILLIPLEX® MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602MAG).
Materials Required but Not Delivered • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
References
Product Information
Detection method Luminex xMAP
Configuration Single Plex
Panel Type Cell Signaling
Applications
Application Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
Key Applications
  • Multiplexing
Application Notes • An overnight (4°C) incubation is recommended for best results
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
Biological Information
Species Reactivity
  • Human
  • Mouse
  • Rat
Analytes Available
  • p38α/SAPK2 (Thr180/Tyr182)
Entrez Gene Number
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Recommended storage for kit components is 2 - 8°C
Packaging Information
Material Size 96-well plate
Transport Information
Supplemental Information
Specifications

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