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Total Erk/MAP Kinase 1/2 Mag MAPmate

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货号：46-609MAG

品牌：Millipore 密理博

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Replacement Information

重要规格表

Analytes Available Species Reactivity Key Applications Detection Methods
Erk/MAPK 1/2 (Total) H, M Mplex Luminex xMAP
Description
Catalogue Number 46-609MAG
Trade Name
  • MILLIPLEX
Description MILLIPLEX MAP Total Erk/MAPK 1/2 Magnetic Bead MAPmate - Cell Signaling Single Plex
Materials Required but Not Delivered • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
References
Product Information
Detection method Luminex xMAP
Configuration Single Plex
Panel Type Cell Signaling
Applications
Application MAPmate single plexes allow you to design your own cell signaling panel. The analyte available for this kit is Erk/MAPK 1/2 Total.
Key Applications
  • Multiplexing
Application Notes • An overnight (4°C) incubation is recommended for best results
• This assay requires 25 µL diluted cell lysate per well
• Please consult the buffer compatibility chart or protocol for appropriate buffer choice
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg)
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • Erk/MAPK 1/2 (Total)
Entrez Gene Number
Entrez Gene Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol
  • MAPK1
  • PRKM2
  • MAPK2
  • p42-MAPK
  • p38
  • ERK
  • P42MAPK
  • ERK2
  • p40
  • PRKM1
  • p41
  • ERK-2
  • ERT1
  • p41mapk
UniProt Number
UniProt Summary FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
COFACTOR: Magnesium (By similarity).
SIZE: 360 amino acids; 41390 Da
SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size 96-well plate
Material Package 96-well Plate
Transport Information
Supplemental Information
Specifications

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