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GP X VAChT

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货号：AB1588

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R IHC, WB Gp Affinity Purified Polyclonal Antibody
Description
Catalogue Number AB1588
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-Vesicular Acetylcholine Transporter Antibody
Alternate Names
  • VAChT
References
Product Information
Format Affinity Purified
Presentation Affinity purified immunoglobulin. Lyophilized from 0.1 M PBS with 1.0% BSA and 0.1% sodium azide. Reconstitute with 100 μL of sterile distilled water.
Applications
Application This Anti-Vesicular Acetylcholine Transporter Antibody is validated for use in IH, WB for the detection of Vesicular Acetylcholine Transporter.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application Notes Immunohistochemistry: 1:500-1:1,500 on 4% paraformaldehyde fixed tissue. Sections were cut at 15 μm with a cryostat or 30 μm with a sliding microtome.

Immunoblotting

Blocking peptide available (Catalog Number AG223).

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Synthetic peptide corresponding to the C-terminus of the predicted rat VAChT protein.
Host Guinea Pig
Specificity Vesicular Acetylcholine Transporter (VAChT). By Western blot the antibody reacted with a single band in rat brain homogenates. This band corresponded to the size of the predicted rVAChT. There was no staining of blots incubated with the antibody that had been preabsorbed with the peptide used for antibody production. By immunohistochemistry the staining was strong in the inner retina where cholinergic processes are located and weak in cholinergic cell bodies. There was no background staining noted. In the CNS staining was strong in fibers and stains cholinergic cell bodies well.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
Gene Symbol
  • SLC18A3
  • MGC12716
  • VACHT
  • VAChT
UniProt Number
UniProt Summary FUNCTION: SwissProt: Q16572 # Involved in acetylcholine transport into synaptic vesicles.
SIZE: 532 amino acids; 56961 Da
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Peripheral and central cholinergic nervous systems.
SIMILARITY: SwissProt: Q16572 ## Belongs to the major facilitator superfamily. Vesicular transporter family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain lyophilized material at 2-8°C for up to 12 months. Maintain reconstituted material at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size 20 µg
Transport Information
Supplemental Information
Specifications

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