Sh X Actin Gamma

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	ICC, IHC, WB	Sh	Serum	Polyclonal Antibody

Species Reactivity

Key Applications

Host

Format

Antibody Type

H, M, R

ICC, IHC, WB

Serum

Polyclonal Antibody

Description
Catalogue Number	AB3265
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Actin γ Antibody
Background Information	Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}

Description

Catalogue Number

AB3265

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Anti-Actin γ Antibody

Background Information

Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}

Product Information
Format	Serum
Control	Smooth muscle
Presentation	Serum. Liquid, containing no preservatives.

Product Information

Format

Serum

Control

Smooth muscle

Presentation

Serum. Liquid, containing no preservatives.

Applications
Application	Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB.
Key Applications	Immunocytochemistry Immunohistochemistry Western Blotting
Application Notes	Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis. Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol. Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used. Optimal working dilutions must be determined by the end user.

Applications

Application

Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB.

Key Applications

Immunocytochemistry
Immunohistochemistry
Western Blotting

Application Notes

Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis.

Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol.

Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used.

Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.
Epitope	gamma
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Sheep
Specificity	Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001614.2
Entrez Gene Summary	Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
Gene Symbol	ACTG1 ACTB Gamma-actin DFNA26 ACTG DFNA20 ACT
Purification Method	Unpurified
UniProt Number	P63261
UniProt Summary	FUNCTION: SwissProt: P63261 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. SIZE: 375 amino acids; 41793 Da SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. DISEASE: SwissProt: P63261 # Defects in ACTG1 are the cause of non-syndromic sensorineural deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. SIMILARITY: SwissProt: P63261 ## Belongs to the actin family. MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.
Molecular Weight	42-43kDa

Biological Information

Immunogen

Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.

Epitope

gamma

Concentration

Please refer to the Certificate of Analysis for the lot-specific concentration.

Host

Sheep

Specificity

Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.

Species Reactivity

Human
Mouse
Rat

Antibody Type

Polyclonal Antibody

Entrez Gene Number

NM_001614.2

Entrez Gene Summary

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.

Gene Symbol

ACTG1
ACTB
Gamma-actin
DFNA26
ACTG
DFNA20
ACT

Purification Method

Unpurified

UniProt Number

P63261

UniProt Summary

FUNCTION: SwissProt: P63261 # Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SIZE: 375 amino acids; 41793 Da
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
DISEASE: SwissProt: P63261 # Defects in ACTG1 are the cause of non-syndromic sensorineural deafness autosomal dominant type 20 (DFNA20) [MIM:604717]; also called autosomal dominant deafness type 26 (DFNA26). DFNA20 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
SIMILARITY: SwissProt: P63261 ## Belongs to the actin family.
MISCELLANEOUS: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Molecular Weight

42-43kDa

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Storage and Shipping Information

Storage Conditions

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Packaging Information
Material Size	100 µL

Packaging Information

Material Size

100 µL

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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