APOPTOSIS INDUCER SET PROTOTYPE KIT

Species Reactivity	Key Applications
Ma	ACT

Species Reactivity

Key Applications

ACT

Description
Catalogue Number	APT800
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Apoptosis Inducer Set
Background Information	Actinomycin D: Classical anti-neoplastic drug. Cytotoxic inducer of apoptosis against tumor cells. A DNA dependent inhibitor of RNA synthesis, actinomycin promotes induction of apoptosis by some specific stimuli, for example, TRAIL and Fas (CD95). Actinomycin D can also alleviate or block the apoptotic process and decrease the cytotoxicity induced by several stimuli such as the dihydrofolate reductase inhibitor aminopterin and the prostaglandin derivative 15-deoxy-D12,14-prostaglandin J2, thus it can have both pro and ant-apoptotic activities in some systems. Camptothecin: Powerful anti-tumor agent. Camptoethecin is an inhibitor of DNA-topoisomerase I. The drug activates the p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cancer cells. In addition, Camptoethecin induced apoptosis can be increased by the presence of cytokines such as TNF via its subsequent inhibition of NFkappa B {Valente P et al Eur J Cancer (2003) 39:1468-77}. Cycloheximide: A potent protein translation inhibitor which results in the inhibition of protein synthesis and subsequent arrest of cell growth and induction of cell death. Cycloheximide also facilitates apoptosis induction via FasL, however, cycloheximide can also prevent apoptosis in some systems, such as thalassemic serum induction of HUVEC cells {Banjerdpong, R. (1998) Ph.D Thesis Mahidol Univ. Bangkok, Thailand}. Dexamethasone: Classic inducer of apoptosis in thymocytes; a glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth and differientiation; modulator of nitric oxide synthase. Induces apoptosis in thymocytes as well as other immune cells like microglia. However, like other apoptotic inducers, dexamethasone can suppress apoptosis in certain systems through the activation of anti-apoptotic proteins such as bcl-X {Chang, et al (1997) FEBS Letter 415:11-15}. Etoposide: Potent topoisomerase II inhibitor. An anti-tumor agent that enhances dsDNA & ssDNA cleavage and inhibits DNA religation. Inhibits the cell cycle in the S-phase and induces apoptosis in normal and tumor cell lines, but can be modulated by intracellular levels of cAMP and other factors {Bermejo, L et al (1998) J. Cell Sci 111:637-644;Solovyyan, V et al. (1998) Brain Res Mol Brain Res 62:43-55}

Description

Catalogue Number

APT800

Brand Family

Chemicon®

Trade Name

Chemicon

Description

Apoptosis Inducer Set

Background Information

Actinomycin D: Classical anti-neoplastic drug. Cytotoxic inducer of apoptosis against tumor cells. A DNA dependent inhibitor of RNA synthesis, actinomycin promotes induction of apoptosis by some specific stimuli, for example, TRAIL and Fas (CD95). Actinomycin D can also alleviate or block the apoptotic process and decrease the cytotoxicity induced by several stimuli such as the dihydrofolate reductase inhibitor aminopterin and the prostaglandin derivative 15-deoxy-D12,14-prostaglandin J2, thus it can have both pro and ant-apoptotic activities in some systems.

Camptothecin: Powerful anti-tumor agent. Camptoethecin is an inhibitor of DNA-topoisomerase I. The drug activates the p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cancer cells. In addition, Camptoethecin induced apoptosis can be increased by the presence of cytokines such as TNF via its subsequent inhibition of NFkappa B {Valente P et al Eur J Cancer (2003) 39:1468-77}.

Cycloheximide: A potent protein translation inhibitor which results in the inhibition of protein synthesis and subsequent arrest of cell growth and induction of cell death. Cycloheximide also facilitates apoptosis induction via FasL, however, cycloheximide can also prevent apoptosis in some systems, such as thalassemic serum induction of HUVEC cells {Banjerdpong, R. (1998) Ph.D Thesis Mahidol Univ. Bangkok, Thailand}.

Dexamethasone: Classic inducer of apoptosis in thymocytes; a glucocorticoid anti-inflammatory agent. Regulates T cell survival, growth and differientiation; modulator of nitric oxide synthase. Induces apoptosis in thymocytes as well as other immune cells like microglia. However, like other apoptotic inducers, dexamethasone can suppress apoptosis in certain systems through the activation of anti-apoptotic proteins such as bcl-X {Chang, et al (1997) FEBS Letter 415:11-15}.

Etoposide: Potent topoisomerase II inhibitor. An anti-tumor agent that enhances dsDNA & ssDNA cleavage and inhibits DNA religation. Inhibits the cell cycle in the S-phase and induces apoptosis in normal and tumor cell lines, but can be modulated by intracellular levels of cAMP and other factors {Bermejo, L et al (1998) J. Cell Sci 111:637-644;Solovyyan, V et al. (1998) Brain Res Mol Brain Res 62:43-55}

Product Information
Components	· Actinomycin D (10 mM): 50 μL · Camptothecin (2 mM): 1 mL · Cycloheximide (100 mM): 1 mL · Dexamethasone (10 mM): 1 mL · Etoposide (10 mM): 100 μL
Presentation	Liquid in DMSO containing no preservatives.

Product Information

Components

· Actinomycin D (10 mM): 50 μL
· Camptothecin (2 mM): 1 mL
· Cycloheximide (100 mM): 1 mL
· Dexamethasone (10 mM): 1 mL
· Etoposide (10 mM): 100 μL

Presentation

Liquid in DMSO containing no preservatives.

Applications
Application	Apoptosis Inducer Set contains Actinomycin D, Camptothecin, Cycloheximide, Dexamethasone & Etoposide. These compounds can be used for inducing apoptotic activity in many cell culture systems.
Key Applications	Activity Assay
Application Notes	It is recommended to use 1000X dilutions of the supplied stocks as a starting point for inducing apoptotic activity in many cell culture systems (e.g., Add 1 μL to 1 mL culture medium), however, the optimal doses may vary for different cell and culture conditions. For small volumes diluting the stocks 1:10 into fresh DMSO, then diluting the material further 1:100 into the culture media or PBS can facilitate the addition of the inhibitor to small volumes of less than 1 mL.

Applications

Application

Apoptosis Inducer Set contains Actinomycin D, Camptothecin, Cycloheximide, Dexamethasone & Etoposide. These compounds can be used for inducing apoptotic activity in many cell culture systems.

Key Applications

Activity Assay

Application Notes

It is recommended to use 1000X dilutions of the supplied stocks as a starting point for inducing apoptotic activity in many cell culture systems (e.g., Add 1 μL to 1 mL culture medium), however, the optimal doses may vary for different cell and culture conditions. For small volumes diluting the stocks 1:10 into fresh DMSO, then diluting the material further 1:100 into the culture media or PBS can facilitate the addition of the inhibitor to small volumes of less than 1 mL.

Biological Information
Species Reactivity	Mammals

Biological Information

Species Reactivity

Mammals

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Store kit materials at -20°C for up to their expiration date. Avoid repeated freeze/thaw cycles. Protect product tubes from light. WARNING: Kit contains highly toxic agents; professional users only; wear gloves and other protective equipment and good laboratory practices when manipulating the concentrated and diluted solutions.

Storage and Shipping Information

Storage Conditions

Store kit materials at -20°C for up to their expiration date. Avoid repeated freeze/thaw cycles. Protect product tubes from light.

WARNING: Kit contains highly toxic agents; professional users only; wear gloves and other protective equipment and good laboratory practices when manipulating the concentrated and diluted solutions.

Packaging Information
Material Size	1 kit

Packaging Information

Material Size

1 kit

实验耗材

分子生物学试剂

蛋白与免疫学

生化试剂

细胞及检测试剂

色谱及层析

仪器设备

实验服务

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APOPTOSIS INDUCER SET PROTOTYPE KIT

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