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Chk X MAP2

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货号：AB5543

品牌：Millipore 密理博

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重要规格表

Species Reactivity Key Applications Host Format Antibody Type
R ICC, IHC, WB Ch Purified Polyclonal Antibody
Description
Catalogue Number AB5543
Brand Family Chemicon®
Trade Name
  • Chemicon
Description Anti-MAP2 Antibody
References
Product Information
Format Purified
Control
  • Positive = cerebral cortex. Negative = liver or kidney.
Presentation Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.
Applications
Application Anti-MAP2 Antibody is an antibody against MAP2 for use in IC, IH & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blotting
Application Notes Western blot: 1:5,000-1:10,000 using ECL. Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.

Immunohistochemistry on frozen tissue sections. Suggested fixative is 4% paraformaldehyde (1-10 minutes) followed by 5 minutes in -20°C methanol.

Immunocytochemistry: 1:5,000-1:10,000 on cells in tissue culture fixed for 1-10 minutes in 10% formalin followed by five minutes in -20°C methanol.

Optimal working dilutions must be determined by the end user.
Biological Information
Immunogen Purified bovine brain MAP2
Concentration Please refer to the Certificate of Analysis for the lot-specific concentration.
Host Chicken
Specificity Microtubule Associated Protein 2 (MAP2). It is expected that the antibody will react with all mammals.
Species Reactivity
  • Rat
Antibody Type Polyclonal Antibody
Entrez Gene Number
Entrez Gene Summary This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
Gene Symbol
  • MAP2
  • MAP2B
  • MAP-2
  • MAP2C
  • DKFZp686I2148
  • MAP2A
Purification Method Ammonium Sulfate Precipitation
UniProt Number
UniProt Summary FUNCTION: SwissProt: P11137 # The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SIZE: 1827 amino acids; 199539 Da
PTM: MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: P11137 ## Contains 3 Tau/MAP repeats.
Molecular Weight Recognizes a doublet of ~280 kDa and a doublet of ~70kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size 50 µL
Transport Information
Supplemental Information
Specifications

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